In order to evaluate the therapeutic effect of dehydropachymic acid(DPA)on eczema rats and its effect on immune response,a rat model of eczema induced by dinitrochlorobenzene(DNCB)was established.Different concentrations of DPA(10,40,and 80 mg/kg)were used to treat eczema rats for fourteen days,and compound glycyrrhizin(CG)(15 mg/kg)was used as the positive treatment control.H-E staining was used to observe the histopathological morphology of rat skin tissues.ELISA was used to detect the levels of serum IL-4 and IFN-y as well as IgA,IgM and IgG.Concanavalin A(ConA)-activated lymphocytes were co-cultured with human keratinocyte HaCaT,followed by treatment of 40 μg/mL DPA.The proliferation of HaCaT cells was detected by MTT method,and the apoptosis of HaCaT cells was detected by TUNEL method.H-E staining outcomes showed that DPA relieved the skin lesions of eczema rats in a dose-dependent manner.DPA decreased the levels of serum IL-4,IgA,IgM and IgG in rats with eczema,and increased the level of IFN-y(all with P＜0.05).The reliefs of eczema in middle-dose(40 mg/kg)and high-dose(80 mg/kg)DPA-treated rats were equivalent to that of 15 mg/kg CG-treated posi-tive control rats.DPA did not affect the proliferation of HaCaT cells(P＞0.05).However,DPA inhibited the apoptosis of HaCaT cells co-cultured with lymphocytes(P＜0.05).Therefore,DPA regulates the immune function of the eczema rats by improving the Th1/Th2 balance,thereby alleviating eczema-related lesions.
eczemadehydropachymic acidhelper T cell 1/helper T cell 2 balanceimmunitykeratinocyte
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