Therapeutic effect of dehydropachymic acid on rats with eczema and its effect on immune response
In order to evaluate the therapeutic effect of dehydropachymic acid(DPA)on eczema rats and its effect on immune response,a rat model of eczema induced by dinitrochlorobenzene(DNCB)was established.Different concentrations of DPA(10,40,and 80 mg/kg)were used to treat eczema rats for fourteen days,and compound glycyrrhizin(CG)(15 mg/kg)was used as the positive treatment control.H-E staining was used to observe the histopathological morphology of rat skin tissues.ELISA was used to detect the levels of serum IL-4 and IFN-y as well as IgA,IgM and IgG.Concanavalin A(ConA)-activated lymphocytes were co-cultured with human keratinocyte HaCaT,followed by treatment of 40 μg/mL DPA.The proliferation of HaCaT cells was detected by MTT method,and the apoptosis of HaCaT cells was detected by TUNEL method.H-E staining outcomes showed that DPA relieved the skin lesions of eczema rats in a dose-dependent manner.DPA decreased the levels of serum IL-4,IgA,IgM and IgG in rats with eczema,and increased the level of IFN-y(all with P＜0.05).The reliefs of eczema in middle-dose(40 mg/kg)and high-dose(80 mg/kg)DPA-treated rats were equivalent to that of 15 mg/kg CG-treated posi-tive control rats.DPA did not affect the proliferation of HaCaT cells(P＞0.05).However,DPA inhibited the apoptosis of HaCaT cells co-cultured with lymphocytes(P＜0.05).Therefore,DPA regulates the immune function of the eczema rats by improving the Th1/Th2 balance,thereby alleviating eczema-related lesions.
eczemadehydropachymic acidhelper T cell 1/helper T cell 2 balanceimmunitykeratinocyte
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