Regulatory effects and mechanisms of miR-409-3p on myocardial injury in young rats with Kawasaki disease
To study the influence of miR-409-3p on myocardial injury in young mice with Kawasaki disease(KD)through silent information regulator 1(SIRT1)/forkhead transcription factor O1(FOXO1)signaling pathway,SD juvenile male rats were randomly divided into control group,model group,miR-409-3p antagomir group,miR-409-3p antagomir negative control group,EX527(SIRT1 inhibitor,1 μg/kg)group,and miR-409-3p antagomir+EX527(1 μg/kg)group,12 per group.The expression of miR-409-3p in rat myocardium was detected by qRT-PCR;ELISA was performed to detect levels of creatine kinase isoenzyme(CK-MB),cardiac troponin Ⅰ(cTnⅠ),cardiac troponin T(cTnT),TNF-α,IL-17,IL-18 in serum.The result showed that compared with the model group and the miR-409-3p antagomir+EX527 group,the pathological injuries in myocardial tissue of the miR-409-3p antagomir group were alleviated,the serum CK-MB,cTnⅠ,cTnT,TNF-α,IL-17,and IL-18 levels,myocardial acely-FOXO1/FOXO1 were decreased(P＜0.05),the cardiac function indexes the left ventricular ejection fraction(LVEF)and the left ventricular fractional shortening(LVFS),and the expression of SIRT1 in myocardial tissue were increased(P＜0.05);the pathological injuries in myocardial tissue of the EX527 group were aggravated,the serum CK-MB,cTnⅠ,cTnT,TNF-α,IL-17,and IL-18 levels,myocardial acely-FOXO1/FOXO1 were increased(P＜0.05),the cardiac function indexes LVEF and LVFS,and the expression of SIRT1 in myocardial tissue were decreased(P＜0.05).miR-409-3p was able to target down-regulation of SIRT1 expression in rat cardiomyocytes.The study suggests that miR-409-3p can target down-regulate SIRT1 expression to participate in the process of myocardial injury in KD young rats.Down-regulation of miR-409-3p expression can play an anti-inflammatory effect by activating SIRT1/FOXO1 signal,thereby reducing myocardial injury in KD young rats.
microRNA-409-3psilent information regulator 1/forkhead transcription factor O1Kawasaki diseaseyoung ratmyocardial injury
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