circ_0000885 regulates the proliferation,apoptosis and migration of glioma cells by targeting miR-186
To investigate the effect of circular RNA 0000885(circ_0000885)on the proliferation,apoptosis and migration of glioma cells,and further to explore its potential mechanism,circ_0000885 and miR-186 expression in normal brain tissues and glioma tissues was analyzed using qRT-PCR.Dual luciferase reporter experiment verified the targeting relationship between circ_0000885 and miR-186.Small interfering RNA negative control(si-NC),circ_0000885 small interfering RNA(si-circ_0000885),miRNA mimic negative control(miR-NC),miR-186 mimic,miRNA inhibitor negative control(anti-miR-NC),miR-186 inhibitor(anti-miR-186),and si-circ_0000885+anti-miR-186 were transfected into glioma cell line LN229.The effects of circ_0000885 and miR-186 expression on cell viability,colony formation,apoptosis and migration of LN229 cells was evaluated by cell counting kit(CCK-8),plate cloning experiment,flow cytometry,and Transwell experiment,respectively.The transplanted tumor volume was detected by the nude mouse xenograft experiment.The results showed that compared to that of the normal brain tissue,circ_0000885 expression in glioma tissue was significantly increased(P＜0.05),while miR-186 expression was significantly decreased(P＜0.05).Interfering with the expression of circ_0000885 or overexpressing miR-186 significantly decreased the viability,colony formation number,and migration number of LN229 cells(P＜0.05),increased cell apoptosis rate and miR-186 expression level(P＜0.05),while inhibiting the expression of miR-186 had the opposite effect.Inhibition of miR-186 expression significantly attenuated the effects of JP2interfering circ_0000885 expression on LN229 cell viability,colony formation,migration,apoptosis and xenograft growth.This study suggests that interfering with circ_0000885 inhibits the tumor proliferation and migration,and induces cell apoptosis of glioma cells by targeting and up-regulating miR-186.
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