Silencing circMET targets miR-30a-5p/ITGβ3 to inhibit the invasion and migration of human rectal cancer cell line SW1463
This study aims to investigate the effect of silencing cyclic ribonucleic acid MET(circMET)on inhibiting the invasion and migration of human rectal cancer cell by targeting miR-30a-5p/integrin β3(ITGβ3).Human rectal cancer cell line SW1463 were divided into si-NC group,si-circMET group,miR-NC group,miR-30a-5p group,si-circMET+anti-miR-NC group,and si-circMET+anti-miR-30a-5p group,with 6 replicates in each group.Transwell assays were used to detect cell invasion and migration.The expressions of circMET,miR-30a-5p,ITGβ3,matrix metalloproteinase 2(MMP-2),and matrix metalloproteinase 9(MMP-9)were determined by qRT-PCR and Western blotting.Dual luciferase reporter gene assays were used to verify the targeting of circMET to miR-30a-5p and miR-30a-5p to ITGβ3,respectively.The results showed that compared to those of the si-NC group,the number of invasive and migrating cells,the expression of circMET,and the mRNA and protein expressions of ITGβ3,MMP-2,and MMP-9 in the si-circMET group were decreased,whereas the expression of miR-30a-5p was increased(P＜0.05).Compared to those of the miR-NC group,the number of invasive and migrating cells,the expression of circMET,and the mRNA and protein levels of ITGβ3,MMP-2,and MMP-9 in the miR-30a-5p group were decreased,while the expression of miR-30a-5p was increased(P＜0.05).Compared to those of the si-circMET+anti-miR-NC group,the number of invasive and migrating cells,the expression of circMET,and the mRNA and protein levels of ITGβ3,MMP-2,and MMP-9 in the si-circMET+anti-miR-30a-5p group were increased,while the expression of miR-30a-5p was decreased(P＜0.05).In addition,Dual luciferase reporter gene assays confirmed the targeted regulatory relationship between circMET and miR-30a-5p as well as miR-30a-5p and ITGβ3.In summary,silencing circMET can inhibit the invasion and migration of human rectal cancer cell line SW1463,which may be achieved by targeting miR-30a-5p to inhibit the expressions of ITGβ3,MMP-2,and MMP-9.
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