Expressions and clinical significance of AhR and IL-22 in patients with systemic lupus erythematosus
This study aims to investigate the protein and mRNA expressions of IL-22 and aryl hydrocarbon receptor(AhR)in the peripheral blood of patients with systemic lupus erythematosus(SLE),and to analyze the correlation between IL-22 and AhR and SLE disease activity index(SLEDAI).The expression levels of IL-22 and AhR protein in the peripheral blood of 43 SLE patients(25 in active stage and 18 in inactive stage)and 41 healthy controls were measured by ELISA.The expression levels of IL-22 and AhR mRNA in PBMC of SLE patients,healthy controls,and 31 rheumatoid arthritis(RA)patients by qRT-PCR.Spearman analysis was used to calculate the correlations of IL-22 and AhR protein and mRNA levels with the SLEDAI in SLE patients.ROC curve was used to analyze the combined diagnostic effect of anti-dsDNA antibody,IL-22,and AhR protein levels in SLE.The results showed that the levels of IL-22,AhR protein and mRNA were higher in SLE patients than those in healthy controls(P＜0.001),and they were all higher in the active phase than in the inactive phase(P＜0.05).IL-22 expressions were significantly elevated in SLE patients compared to RA patients(P＜0.05).Spearman analysis showed that IL-22,AhR protein and mRNA levels were all positively correlated with SLEDAI in SLE patients(P＜0.01).The AUC of combined diagnosis of IL-22,AhR,and anti-dsDNA antibody for SLE was 0.986,with a sensitivity and specificity of 90.7％and 97.6％,respectively.In conclusion,the protein and mRNA levels of IL-22,AhR in SLE patients are significantly higher than those in healthy subjects and are positively correlated with SLEDAI.The diagnosis of serum IL-22 and AhR combined with anti-dsDNA antibody has higher accuracy and may improve the clinical diagnosis of SLE in the future.
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