Study on cGAS/STING gene expression,dendritic cell composition,and matu-ration markers in the immune response of children with adenovirus pneumonia
This study aims to investigate the changes in mRNA expressions of stimulator of interferon gene(STING)and cyclic GMP AMP synthase(cGAS),the composition of myeloid dendritic cells(mDC)and their surface maturation markers CD80 and CD86,the related cytokines,and the correlation among above indicators to analyze the immune response upon adeno-virus pneumonia infection.34 children with acute stage adenovirus pneumonia and 34 healthy children were included in the study.The relative mRNA expression levels of STING and cGAS in the peripheral mononuclear cells were detected by qRT-PCR.The levels of IFN-α,IFN-β,TNF-α,and IL-6 were detected by ELISA.FACS was used to detect the mDC composition and surface markers CD80 and CD86.The correlation of the above indicators was analyzed.The results showed that compared to those of the control group,mRNA expression levels of STING and cGAS,IFN-α,IFN-β,TNF-α,IL-6,mDC cell number,CD80,and CD86 were all increased in adenovirus pneumonia patients(P＜0.05).The level of mDC was positively correlated with the expressions of STING mRNA,cGAS mRNA,IFN-α,TNF-α,and IL-6.The CD80 level was positively correlated with STING mRNA,cGAS mRNA,IFN-α,IFN-β,TNF-α,and IL-6,whereas CD86 was positively correlated with IFN-α,IFN-β,and TNF-α(P＜0.05).This study suggests that STING/cGAS genes are highly expressed in children with adenovirus pneumonia who exhibit increased mDC number,maturation markers,and cytokine secretion.The cGAS-STING pathway may be further activated to mediate innate immunity and exert antiviral effect.
adenovirus pneumoniastimulator of interferon genecyclic GMP AMP synthasemyeloid dendritic cellcytokine
NETL
NSTL
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