Compound fufangteng mixture regulates CD69 and PD-1 expressions of peripheral CD19+B cells in immunosuppressed mice
This study aims to evaluate the immune-enhancing effect of compound fufangteng mixture(CFM)on B cells in cyclo-phosphamide-induced immunosuppression in BALB/c mice.BALB/c mice were randomly divided into normal control(NC)group,model control(MC)group,low-dose CFM(LD)group,medium-dose CFM(MD)group,high-dose CFM(HD)group,and positive control(PD)group.The expressions of CD19+B cell surface activating molecules(CD69,CD38)and exhaustion molecules(PD-1,CD95)in mice peripheral blood and spleen were detected by FACS.The results showed that compared to that of the NC group,the percentage of CD19+B cells in the peripheral blood of MC group decreased significantly(P＜0.05),while the percentages of CD19+B cells in the peripheral blood of LD group,MD group,and HD group increased in a CFM dose-dependent manner.The expressions of CD69 and PD-1 of CD19+B cells in the peripheral blood of LD group and MD group were significantly higher than those of the NC group(P＜0.05).There was no significant difference in the expression levels of CD69,CD38,PD-1,and CD95 of splenetic CD19+B cells among the six groups(P＞0.05).Altogether,the results suggest that the CFM may improve the percentage of peripheral blood CD19+B cells and regulate CD69 and PD-1 in immuno-suppression mice.
compound fufangteng mixtureimmunosuppressionCD19+B cellCD69programmed death 1
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