复方扶芳藤合剂调节免疫抑制小鼠外周血CD19+B细胞CD69和PD-1的表达
Compound fufangteng mixture regulates CD69 and PD-1 expressions of peripheral CD19+B cells in immunosuppressed mice
彭丽珊 1陈果 2李建明 1杨洋 1刘显 1肖健1
作者信息
- 1. 广西中医药大学 广西高发传染病中西医结合转化医学重点实验室,南宁 530001
- 2. 湖北省中医院 脾胃病科,武汉 430060
- 折叠
摘要
为评估复方扶芳藤合剂(compound fufangteng mixture,CFM)对环磷酰胺诱导的免疫抑制BALB/c小鼠B细胞的免疫增强作用,将BALB/c小鼠随机分为空白对照(normal control,NC)组、模型(model control,MC)组、CFM低剂量(low-dose CFM,LD)组、CFM 中剂量(medium-dose CFM,MD)组、CFM 高剂量组(high-dose CFM,HD)组和阳性对照(positive con-trol,PC)组.采用FACS检测小鼠外周血和脾脏CD19+B细胞表面活化分子(CD69、CD38)和耗竭分子(PD-1、CD95)的表达.结果显示,与NC组比较,MC组外周血CD19+B细胞百分比显著下降(P＜0.05),LD组、MD组、HD组外周血CD19+B细胞百分比有改善的趋势,并随着CFM剂量的递增而升高;LD组、MD组外周血CD19+B细胞CD69和PD-1表达水平显著高于NC组(P＜0.05);与外周血CD19+B细胞比较,脾脏中CD19+B细胞CD69、CD38、PD-1和CD95的表达水平在各组间差异无统计学意义(P＞0.05).该研究提示,CFM对小鼠外周血CD19+B细胞可能具有改善作用,且对其CD69和PD-1有调节作用.
Abstract
This study aims to evaluate the immune-enhancing effect of compound fufangteng mixture(CFM)on B cells in cyclo-phosphamide-induced immunosuppression in BALB/c mice.BALB/c mice were randomly divided into normal control(NC)group,model control(MC)group,low-dose CFM(LD)group,medium-dose CFM(MD)group,high-dose CFM(HD)group,and positive control(PD)group.The expressions of CD19+B cell surface activating molecules(CD69,CD38)and exhaustion molecules(PD-1,CD95)in mice peripheral blood and spleen were detected by FACS.The results showed that compared to that of the NC group,the percentage of CD19+B cells in the peripheral blood of MC group decreased significantly(P＜0.05),while the percentages of CD19+B cells in the peripheral blood of LD group,MD group,and HD group increased in a CFM dose-dependent manner.The expressions of CD69 and PD-1 of CD19+B cells in the peripheral blood of LD group and MD group were significantly higher than those of the NC group(P＜0.05).There was no significant difference in the expression levels of CD69,CD38,PD-1,and CD95 of splenetic CD19+B cells among the six groups(P＞0.05).Altogether,the results suggest that the CFM may improve the percentage of peripheral blood CD19+B cells and regulate CD69 and PD-1 in immuno-suppression mice.
关键词
复方扶芳藤合剂/免疫抑制/CD19+B细胞/CD69/程序性死亡受体1Key words
compound fufangteng mixture/immunosuppression/CD19+B cell/CD69/programmed death 1引用本文复制引用
基金项目
国家自然科学基金(81860780)
广西自然科学基金面上项目(2020GXNSFAA297162)
广西壮族自治区中医药管理局基金项目(GXZYA20220087)
出版年
2024
NETL
NSTL
万方数据