miR-29c-3p inhibits proliferation,migration,and invasion of cholangio-carcinoma cells by targeting PLAU
In order to explore the mechanism of miR-29c-3p on the proliferation,migration,and invasion of cholangiocarcinoma cells by targeting urokinase-type plasminogen activator(PLAU),HuCCT1 and HCCC9810 cell lines with stable overexpression of miR-29c-3p were constructed and divided into miR-NC group,miR-29c-3p group,and miR-29c-3p+PLAU group.The differential expressions of miR-29c-3p and PLAU and their relationships with malignant clinical phenotypes of cholangiocarcinoma were analyzed by bioinformatics.The mRNA and protein expressions of miR-29c-3p and PLAU in each group were detected by RT-PCR and Western blotting,respectively.The proliferation,migration,and invasion of cells were detected by CCK-8 and Transwell assay,respectively.The target gene of miR-29c-3p was predicted by bioinformatics,and verified by dual luciferase reporter gene assay.The xenograft models of cholangiocarcinoma nude mice were constructed and divided into miR-NC group and miR-29c-3p group.The volume and mass of tumors were recorded.The results showed that the expression of miR-29c-3p was down-regulated in cholangiocarcinoma.Overexpression of miR-29c-3p inhibited the proliferation,migration,and invasion of cholangiocarcinoma cells,and the growth of xenografted tumor in nude mice(P＜0.05).The expression of PLAU was up-regulated in cholangiocarcinoma,and abnormal expression of PLAU was correlated with malignant clinical phenotypes.Compared to that of the miR-NC group,the expression of miR-29c-3p was increased in miR-29c-3p group(P＜0.05).Bioinfor-matics predicted that PLAU was the target gene of miR-29c-3p,which was verified by dual luciferase reporter gene assay.miR-29c-3p inhibited the proliferation,migration,and invasion of cholangiocarcinoma cells by targeting PLAU(P＜0.05).This study suggests that miR-29c-3p can inhibit the proliferation,migration,and invasion of cholangiocarcinoma cells by negatively regulating the expression of PLAU.
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