Gene Expression Analysis of RNA Helicase Gene SIDEAD34 under Low Temperature Stress in Tomato
Bioinformatics and quantitative RT-PCR were employed to study the protein structure of SlDEAD34 and the expression pattern of SlDEAD34 in different vegetative organs under low temperature stress in tomato.Results showed that SlDEAD34,highly homologous with Arabidopsis LOS4,is a DEADbox RNA helicase with 9 conserved motifs.SlDEAD34 expresses in all tested tissues of 6 weeks seedling,with the highest level in root and lowest in leaf,whereas with the highest level in fruit and lowest in root of 5 months mature plants.Interestingly,the expression levels of SlDEAD34 in root were much lower than in leaf under the low temperature stress of 4 ℃.However,the gene expression of SlDEAD34 has no obvious change in both root and leaf under 12 ℃ treatment in tomato.In conclusion,SlDEAD34 was downregulated significantly after low temperature 4 ℃ treatment.Taken together,we speculated that SlDEAD34 might be involved in the response of tomato to low temperature stress.
tomato (Solanum lycopersicum)RNA helicaseSlDEAD34 genelow temperature stressgene expression
NETL
NSTL
万方数据
维普
