A rapid method to determine the residues of enrofloxacin in fishery products by microbiological chromotest
By screening the sensitive strain and the color developing reagent, we established a microbiological chromotest method to determine the residues of enrofloxacin in the fishery products. Sarcina ureae treated as the indication strain and triphenyl tetrazolium chloride as the color development reagent, the positive samples show red while the negative ones colorless. The detection limits of the method can reach 50 g/kg. This method is precise and reliable, which is verified by Enzyme Linked Immunosorbent Assay (ELISA) and High Performance Liquid Chromatography (HPLC) methods. The detection limits of ELISA method can reach 12 μg/kg while the recovery rates covered 74.25%-93.38%. The detection limits of HPLC method can reach to 5.0 μg/kg while the recovery rates covered 78.20%-91.27%. Compared to the traditional method, this method is more rapid, convenient and easier. It is beneficial to the current procedure and can be applied in these fields such as cultivation, sale, transportation, consumption and quality inspection.
国家科技期刊平台
NSTL
万方数据
维普
