Comparison of growth performance and analysis of genetic structure of 5 specialized strains of Macrobrachium rosenbergii
Growth performance of F2 of 5 specialized strains of Macrobrachium rosenbergii (A,B,C,D and E)was checked by one-way completely randomized design and polyculture in concrete ponds with the fluorescent tag.The results indicated that the ranks of AGR and SGR were D > B > E > C > A in either one-way completely randomized experiment or polyculture in concrete ponds with the fluorescent tag.AGR and SGR by ANOVA and Duncan for strains of multi-average revealed that F2 of B and D were strains with the best growth traits.Every proportion of body form of F2 was greater than F1 ; Variation coefficient of body weight of F2 was lower than F1,the most obvious decline in the comparison of F2 and F1 was B,decreasing 49.22%.All of them suggested preliminary benefit of strain selecting.Microsatellite markers were used to analyze the genetic diversity and genetic differentiation of F2 of 5 specialized strains,including 630 individuals.The results showed the allele number was 6.25; average effective allele number was 3.666 2; average observed heterozygosity value was O.435 0 ; the average expected heterozygosity value was 0.707 6.All of these five specialized strains in the ascending order the PIC was C(0.434 0) < A(0.480 4) < D(0.513 4) < B (0.519 3) < E(0.555 7),which indicated a moderate or high level of polymorphism.The average genetic differentiaton coefficient at each locus was 0.074 5,and the majority of genetic variation was attributed to diference of individuals within specialized strains,suggesting that each specialized strain also has some potential for further selective breeding.