The effects of the loser experience induces anxiety-like behavior through the serotonin pathway on Chinese mitten crab(Eriocheir sinensis)
Agonistic behavior is a common phenomenon in the culture of Eriocheir sinensis.To explore the adverse effects of loser experience,anxiety-like behavior and feeding behavior of E.sinensis after loser experience were observed,and the levels of serotonin(5-HT)and 5-HT7R gene relative expression were detected.Then,the effects of exogenous 5-HT,fluoxetine,and 5-HT7R agonists and antagonists on the anxiety-like behavior of E.sinensis were observed.The results indicated that the E.sinensis that experienced the fight failure showed anxiety-like behavior,which was accompanied by the increase of plasma 5-HT concentration and the decrease of 5-HT7R gene relative expression level in the thoracic ganglion,and the food intake decreased after the loser experience.Exogenous 5-HT treatment promoted anxiety-like behavior,while fluoxetine treatment alleviated anxiety-like behavior.In addition,treatment with 5-HT7R agonist(AS19)promoted anxiety-like behavior,while treatment with 5-HT7R antagonist(SB-269970)alleviated anxiety-like behavior.These results indicate that the loser experience can lead to anxiety-like behavior and affect anxiety-like behavior through the serotonin pathway.This study revealed the potential negative effects of loser experience and provided theoretical basis for promoting healthy culture of E.sinensis.
国家科技期刊平台
NSTL
万方数据
