首页|叶用莴苣(Lactuca sativa)花青素提取技术的优化

叶用莴苣(Lactuca sativa)花青素提取技术的优化

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以紫晶生菜为原料,柠檬酸水溶液为提取剂,分别研究了提取液浓度、浸提温度、固液比、浸提时间和浸提次数对花青素提取率的影响.在此基础上,采用三水平四因素安排实验,以520 nm为最大吸收波长,以吸光度为响应值,探讨浸提温度、浸提时间、提取液浓度和固液比对紫晶生菜花青素提取的影响,并对提取工艺进行优化.筛选出的最佳提取工艺条件为:提取剂质量浓度0.06 g/mL,浸提温度70℃,固液比1/70,浸提时间2h,滤渣提取次数2次.
Optimization of technique for extracting anthocyanins from lettuce (Lactuca sativa)
We investigated the effects of extraction mass concentration,liquid-to-solid rate,temperature,and extraction time and frequency on the extraction rate of anthocyanin from purple lettuce by using citric acid solution as the extraction agent.Then,the orthogonal test of four factors and three levels were performed with the maximum absorption wavelength for 520 nm,and absorbance as response value.The effects of extraction temperature,extraction time,extract mass concentration and solid-liquid ratio on the extraction rate of anthocyanin were discussed through orthogonal test,and the optimum technique for extracting lettuce was obtained.The result showed the optimal extraction condition was as follows:citric acid mass concentration 0.06 g/mL,extraction temperature 70 ℃,ratio of material-liquid 1/70,extraction time 2 h,and extraction 2 times.

lettucecitric acidextractionanthocyaninsextraction process

郁征宇、梁桂逢、周龙宇、徐晨曦、王全华、蔡晓锋

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上海师范大学生命与环境科学学院植物种质资源开发协同创新中心,上海200234

生菜 柠檬酸 提取 花青素 提取工艺

国家自然科学基金青年基金上海市协同创新中心项目上海植物种质资源工程技术研究中心项目

31501754ZF120517DZ2252700

2017

上海师范大学学报(自然科学版)
上海师范大学

上海师范大学学报(自然科学版)

影响因子:0.255
ISSN:1000-5137
年,卷(期):2017.46(5)
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