Identification and expression analysis of HD-ZIP Ⅲ gene family in spinach
During the growth and development of plant leaves,the establishment and maintenance of paraxial and abaxial polarity are essential for the normal functioning of photosynthesis,respiration and transpiration.HD-ZIP Ⅲ genes play a crucial role in establishing leaf adaxial polarity.In this study,three HD-ZIP Ⅲ family genes were identified in the spinach genome,and designated as SoHBl,SoHB2 and SoHB3.Their physicochemical properties,conserved domains and evolutionary relationships were analyzed.The results showed that all three HD-ZIP Ⅲ proteins possessed four conserved domains.Collinear analysis indicated that spinach SoHBl,SoHB2 and SoHB3 exhibited collinear relationships with Arabidopsis ATHB8,ATREV and ATHB15 respectively.The prediction results of promoter cis-acting elements reveled that the promoters of spinach HD-ZIP Ⅲfamily genes contained cis-acting elements related to light response,stress response and hormone response.Analysis of expression patterns in spinach germplasms with different leaf types indicated that the single halberd leaf exhibited the highest expression of HD-ZIP Ⅲ.Furthermore,the expression levels of SoHBl,SoHB2 and SoHB3 genes decreased following drought stress treatment.Expression analysis across various parts of spinach,including roots,stems,bolting leaves,functional leaves,bolting petioles,functional petioles and others,showed that SoHB1 was predominantly expressed in roots,while SoHB2 and SoHB3 were predominantly expressed in bolting leaves.
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