菠菜HD-ZIP Ⅲ基因家族鉴定与表达分析
Identification and expression analysis of HD-ZIP Ⅲ gene family in spinach
刘梓涵 1张琪悦 1袁满 1翁莉 1邵锋 2顾立君 2蔡晓锋1
作者信息
- 1. 上海师范大学生命科学学院,上海 200234
- 2. 上海市崇明区蔬菜科学技术推广站,上海 202150
- 折叠
摘要
植物叶片在生长发育过程中,近-远轴极性的建立与维持是叶片正常行使光合效应、呼吸作用、蒸腾作用的结构基础.HD-ZIP Ⅲ是重要的叶片近轴极性基因,本研究在菠菜(Spinacia oleracea)基因组中鉴定到3个HD-ZIP Ⅲ基因家族成员,依次命名为SoHB1,SoHB2和SoHB3,并对其蛋白理化性质、保守结构域和进化关系进行了分析.研究结果表明:3个HD-ZIP Ⅲ蛋白都具有4个保守结构域.共线性分析表明:菠菜SoHB 1,SoHB2和SoHB3分别与拟南芥ATHB8,ATREV和ATHB15存在共线性关系.启动子顺式作用元件预测结果表明:菠菜HD-ZIP Ⅲ家族基因启动子上游包含光响应、逆境胁迫反应、激素响应等顺式作用元件.在不同叶型菠菜种质中的表达模式分析结果表明:单戟型叶片的SoHBs表达量最高;此外,经干旱胁迫处理后,SoHB1,SoHB2和SoHB3的基因表达量普遍下降;对菠菜包括根、茎、薹叶、功能叶、薹叶柄、功能叶柄等部位进行表达分析,发现SoHB1在根部表达最为丰富,SoHB2和SoHB3在薹叶处表达最为丰富.
Abstract
During the growth and development of plant leaves,the establishment and maintenance of paraxial and abaxial polarity are essential for the normal functioning of photosynthesis,respiration and transpiration.HD-ZIP Ⅲ genes play a crucial role in establishing leaf adaxial polarity.In this study,three HD-ZIP Ⅲ family genes were identified in the spinach genome,and designated as SoHBl,SoHB2 and SoHB3.Their physicochemical properties,conserved domains and evolutionary relationships were analyzed.The results showed that all three HD-ZIP Ⅲ proteins possessed four conserved domains.Collinear analysis indicated that spinach SoHBl,SoHB2 and SoHB3 exhibited collinear relationships with Arabidopsis ATHB8,ATREV and ATHB15 respectively.The prediction results of promoter cis-acting elements reveled that the promoters of spinach HD-ZIP Ⅲfamily genes contained cis-acting elements related to light response,stress response and hormone response.Analysis of expression patterns in spinach germplasms with different leaf types indicated that the single halberd leaf exhibited the highest expression of HD-ZIP Ⅲ.Furthermore,the expression levels of SoHBl,SoHB2 and SoHB3 genes decreased following drought stress treatment.Expression analysis across various parts of spinach,including roots,stems,bolting leaves,functional leaves,bolting petioles,functional petioles and others,showed that SoHB1 was predominantly expressed in roots,while SoHB2 and SoHB3 were predominantly expressed in bolting leaves.
关键词
菠菜(Spinacia/oleracea)/HD-ZIP/Ⅲ基因家族/干旱胁迫/基因表达量Key words
spinach(Spinacia oleracea)/HD-ZIP Ⅲ gene family/drought stress/gene expression引用本文复制引用
基金项目
上海市科技创新行动计划农业科技领域项目(21N11900500)
出版年
2024
NETL
NSTL
万方数据