In this study,CRISPR/Cas9 gene editing technology was used to design four knockout targets located in the promoter region,5'UTR region and exons 2 and 3 of the granule bound starch synthase gene(Waxy,Wx)in the japonica rice variety"Jiahua 1".We then obtained four different types of T1 generation homozygous edited-mutants without transgene to investigate their quality and agronomic traits.Compared to the wild type"Jiahua 1",the amylose content(AC)of the wxpro mutants in the promoter region decreased to 14.09％,while the wxutr mutants in the 5'UTR region decreased to 11.76％,with a loose arrangement of starch grains.The wxex2 and wxex3 mutants in the exon regions had an AC of to less than 2％,similar to that of glutinous rice.In summary,all stable-inherited homozygous wx mutants,with varying AC,not only enrich the Wx allelic mutant genes in rice,but also provide valuable genetic and breeding materials for improving rice eating quality.
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