In this work,a phenol-acclimated sludge(PAS)was obtained by acclimating activated sludge with phenol as electron donor.When phenol was used as electron donor for denitrification,the denitrification rate was as 3.2 folds as that with glucose-acclimated sludge(GAS).The high-throughput sequencing results for the two denitrifying sludges indicate that Chao l index in PAS was higher than that in GAS,but Shannon index in GAS was higher than that in PAS.This suggests that species richness in PAS was higher than that in GAS,but species diversity in PAS was lower than that in GAS.The analysis for the microbial structure and relative abundance of two types of denitrifying sludge documented that the abundance of genus Denitratisoma in PAS is much higher than that in GAS,which let PAS be much superior to GAS in the denitrification with phenol as electron donor,because genus Denitratisoma had both the function of degrading phenol and denitrification.
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