上海师范大学学报(自然科学版)2024,Vol.53Issue(4) :564-570.DOI:10.20192/j.cnki.JSHNU(NS).2024.04.014

一株反硝化惠氏副球菌的分离鉴定及脱氮特性研究

Isolation,identification,and the denitrification characteristics for a denitrifying strain:Paracoccus huijuniae

黄猛 陈松筠 张海雲 高晓冰 张永明
上海师范大学学报(自然科学版)2024,Vol.53Issue(4) :564-570.DOI:10.20192/j.cnki.JSHNU(NS).2024.04.014

一株反硝化惠氏副球菌的分离鉴定及脱氮特性研究

Isolation,identification,and the denitrification characteristics for a denitrifying strain:Paracoccus huijuniae

黄猛 1陈松筠 1张海雲 1高晓冰 1张永明1
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作者信息

  • 1. 上海师范大学环境与地理科学学院,上海 200234
  • 折叠

摘要

在城镇生活污水处理中,氮(N)的高效去除已成为一个重要的内容.其中反硝化是脱氮的关键步骤.研究筛选了一株惠氏副球菌(Paracoccus huijuniae),并通过生物强化的方法提高其反硝化的速率.以普通反硝化污泥作为对照,将P.huijuniae加入到普通硝化污泥进行生物强化,在相同操作条件下,NO3--N的去除速率可以提高34%.高通量测序结果表明:由于P.huijuniae的加入,具有反硝化功能的微生物菌属的丰度得到明显提高.对P.huijuniae的全基因组测序可知,P.huijuniae包含有多个反硝化的关键基因,正是因为这些基因的加入,使反硝化速率明显加速.

Abstract

Nitrogen(N)removal become an important topic in municipal wastewater treatment,in which denitrification seems more important step.A denitrifying strain,Paracoccus huijuniae,was isolated and was employed for accelerating denitrification by means of bioaugmentation.When P.huijuniae was added into common denitrifying sludge for denitrification,NO3--N removal rate was increased by 34%compared with common denitrifying sludge.The results of high throughput sequencing indicated that abundance of genera with denitrification function increased obviously after P.huijuniae was added into common denitrifying sludge.Meanwhile,the whole genome sequencing indicated that P.huijuniae contains multiple key genes for denitrification.This led a significant acceleration for denitrification rate.

关键词

惠氏副球菌(Paracoccus/huijuniae)/反硝化/生物强化

Key words

Paracoccus huijuniae/denitrification/bioaugmentation

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出版年

2024
上海师范大学学报(自然科学版)
上海师范大学

上海师范大学学报(自然科学版)

影响因子:0.255
ISSN:1000-5137
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