Analysis on the relationship between mitochondrial related genes and prognosis of bladder cancer based on TCGA database
Objective To construct a model based on mitochondrial related genes and the prognosis of bladder cancer,and to analyze the relationship between the regression model and the prognosis of bladder cancer.Methods The bladder cancer transcriptome data and clinical information were downloaded from the TCGA,and the R software was used for data processing and analysis.Firstly,the differential expression of mitochondrial related genes in cancer and paracancerous tissues of bladder cancer patients were analyzed.Secondly,the differentially expressed genes related to mitochondria were analyzed by GO enrichment analysis and KEGG signal pathway enrichment analysis.Furthermore,the coxph function in the survival package was used to construct the COX model,and the patients were divided into high and low risk groups according to the median risk score.Kaplan-Meier(K-M)survival analysis was performed.Finally,survival package was used to analyze the relationship between related genes and prognosis of bladder cancer.Finally,GSE48075 data is used to validate the model.Results Among 88 differentially expressed mitochondrial related genes,26 ones were found to be related to prognosis.The genes with high correlation were further eliminated and thirteen independent prognostic genes of bladder cancer were obtained.A cox regression model was established based on the 13 differentially expressed genes.Patients with bladder cancer were divided into high and low risk groups according to risk score.It was found that the survival time of high risk group was significantly lower than that of low risk group.Finally,the model is applied to the verification set data(GSE48075),and it is found that the model can better predict the prognosis of bladder cancer.Conclusion Based on the analysis of TCGA database,the genes related to the prognosis of bladder cancer were screened.A prognostic model of bladder cancer based on the expression level of 13 mitochondrial differential genes was successfully constructed.Verification analysis found that the model can accurately predict the prognosis of patients with bladder cancer.Therefore,the model can provide reference for the prognosis of patients with bladder cancer.
万方数据
维普
