Determination of α-hydroxy acids in cosmetics by UHPLC-MS/MS
[Objective]To establish a UHPLC-MS/MS quantitative method for the determination of glucuronic acid,tartaric acid,glycolic acid,malic acid,lactic acid,citric acid,DL-2-hydroxybutyric acid sodium,mandelic acid,benzilic acid,hydroxycaprylic acid,lactobionic acid,gluconic acid and N-acetylneuraminic acid in cosmetics.[Methods]Samples were prepared by ultrasonic extraction,cleansed by precipitating reagent and followed by high-speed centrifugation of the extraction solution.The supernatant was filtered by 0.22 μm Millipore filter.The continued filtrate was taken for analysis.A reversed phase column,Poroshell 120 EC-C18(2.7 μm,4.6 mm×1 000 mm)was used with 0.1%formic acid buffer and acetonitrile as the mobile phase under the condition of gradient elution.The analytes were detected with electrospray ionization source in negative ion mode(ESI-)and multiple reactions monitoring(MRM),and quantified by external standard curve.[Results]The method showed a good linearity of glucuronic acid,tartaric acid,malic acid,DL-2-hydroxybutyric acid sodium,benzilic acid,hydroxycaprylic acid and N-acetylneuraminic acid within the concentration range of 50.0‒2 000.0 μg·L-1(r>0.995).The method showed a good linearity of glycolic acid,lactic acid,citric acid and mandelic acid within the concentration range of 100.0‒5 000.0 μg·L-1(r>0.995).The method showed a good linearity of lactobionic acid and gluconic acid within the concentration range of 50.0‒5 000.0 μg·L-1(r>0.995).The recoveries were in the range of 92.3%‒114.1%;the relative standard deviations(RSD)were in the range of 0.9%‒6.0%(n=3).The detection limits of glucuronic acid,tartaric acid,malic acid,citric acid,DL-2-hydroxybutyric acid sodium,mandelic acid,benzilic acid,hydroxycaprylic acid,lactobionic acid,gluconic acid and N-acetylneuraminic acid were 0.003%while the detection limits of glycolic acid,lactic acid and mandelic acid were 0.006%.In 10 batches of commercially available cosmetics,eight batches showed positive result.[Conclusion]The UHPLC-MS/MS method is efficient,sensitive and accurate and is applicable to the determination of 13 α-hydroxy acidic components in cosmetics.
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万方数据
维普
