As one of the three-dimensional(3D)cell culture technologies,the 3D scaffold-free culture technology is widely used in the construction of tumor 3D cell models.It is important to understand the current application of tumor cell 3D scaffold-free culture techniques and the results of tumor 3D cell model construction for the development and evaluation of anti-tumor drugs.This article introduces the ultra-low attachment plates culture,hanging drop technique,magnetic levitation culture and rotary cell culture system for constructing a 3D cell model of tumors and their limitations,and outlines some new progress of the application of 3D cell models in the pharmacodynamic evaluation so as to provide reference for the construction of 3D cell models of tumors and the efficacy evaluation of anticancer drugs.
关键词
3D肿瘤细胞模型/无支架细胞培养技术/药效评价
Key words
3D tumor cell model/scaffold-free cell culture techniques/pharmacodynamic evaluation
维普
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