上海医药2024，Vol.45Issue(3) ：71-75.

HPLC法同时测定健腰密骨片浸膏粉中5种指标成分

Simultaneous determination of five constituents in Jianyao Migu tablets by HPLC

商晓慧 赵晓玲 詹常森
上海医药2024，Vol.45Issue(3) ：71-75.
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  • 万方数据

HPLC法同时测定健腰密骨片浸膏粉中5种指标成分

Simultaneous determination of five constituents in Jianyao Migu tablets by HPLC

商晓慧 1赵晓玲 1詹常森1
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作者信息

  • 1. 上海和黄药业有限公司/上海中药固体制剂创新工程技术研究中心 上海 201401
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摘要

目的:建立健腰密骨片浸膏粉的多成分定量分析方法,为评价浸膏粉中间体的质量提供依据.方法:采用HPLC法,以乙腈-磷酸盐缓冲液为流动相,梯度洗脱,测定健腰密骨片浸膏粉中 5 种成分的含量.结果:木兰花碱、丹酚酸B、朝藿定C、淫羊藿苷和宝藿苷Ⅰ5 种成分在各自质量浓度范围内呈良好线性关系,r≥0.999 7;5 种化合物的平均回收率分别为 100.47%、104.37%、95.38%、95.93%、96.66%.该方法的专属性、重复性均良好.结论:该分析方法准确、重复性好,可作为健腰密骨片浸膏粉的质量控制方法.

Abstract

Objective:To establish a multi-component quantitative analysis method for the extract powder of Jianyao Migu tablets and provide a basis for comprehensive evaluation of the quality of extract powder.Methods:The contents of five components in the extract powder of Jianyao Migu tablets were determined by HPLC method with acetonitrile-phosphate buffer as mobile phase and gradient elution.Results:The five components including magnoflorine,salvianolic acid B,epimedin C,icariin and baohuosideⅠshowed a good linear relationship in their respective concentration ranges(r≥0.999 7).The average recovery rates of 5 compounds were 100.47%,104.37%,95.38%,95.93%and 96.66%,respectively.The specificity and reproducibility of this method were good.Conclusion:The established analytical method is accurate and reproducible,and can be used for the quality control of extract powder in Jianyao Migu tablets.

关键词

健腰密骨片浸膏粉/HPLC/多成分定量分析

Key words

extract powder of Jianyao Migu tablets/HPLC/multi-component quantitative analysis

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基金项目

上海市2021年度"科技创新行动计划"生物医药科技支撑专项项目(21S21903100)

出版年

2024
上海医药
上海医药行业协会

上海医药

影响因子：0.781
ISSN：1006-1533
参考文献量12
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