Study on the role of CARD6 in the sepsis-induced myocardial injury
Objective:To explore the regulatory mechanism of CARD6 in sepsis-induced myocardial injury.Methods:C57BL/6 mice were randomly divided into a control group and a sepsis model group.A mouse model of sepsis-induced myocardial injury was constructed using LPS intraperitoneal injection method,and the mRNA expression levels of CARD6 and caspase-1 in mouse myocardial tissue were detected by RT-qPCR.In vitro,H9C2 cells were stimulated by LPS and transfected with overexpression plasmid containing CARD6,and the levels of caspase-1,cell viability and CK-MB content were detected.Results:Compared with the control group,the contents of LDH and CK-MB in myocardial tissue of mice increased in the model group,suggesting that sepsis can cause myocardial damage in mice.In addition,the expression level of CARD6 in the cardiac tissue of the model group was significantly reduced.The intracellular CARD6 expression level in the LPS-treated H9C2 cell also showed a downstream trend.After transfection with the overexpression plasmid containing CARD6,the release of CK-MB levels in H9C2 cells decreased,cell viability increased and the expression level of caspase-1 decreased.Conclusion:CARD6 can ameliorate myocardial injury caused by sepsis and its mechanism may be related to caspase-1 mediated pyroptosis.
万方数据
维普
