目的:体内外评价紫归润唇膏的抗炎功效.方法:建立脂多糖诱导的RAW 264.7 细胞炎症模型,检测紫归醇提物对一氧化氮生成的影响.建立卡泊三醇诱导的特应性皮炎样小鼠模型,研究紫归润唇膏对小鼠耳皮肤厚度、耳皮肤炎症的严重程度、组织病理及安全性的影响.结果:25～200 μg/mL紫归醇提物能够促进RAW 264.7 细胞生长且显著降低一氧化氮水平.同时紫归润唇膏能够显著降低特应性皮炎样小鼠耳部厚度,降低湿疹面积及严重度指数(eczema area and severity index,EASI)评分,减少炎症细胞浸润,降低棘层增厚,改善小鼠耳廓皮损炎症情况,且对卡泊三醇诱导的特应性皮炎样小鼠肝脾肾无影响.结论:紫归润唇膏具有显著抗炎功效且具有安全性.
Evaluation of the anti-inflammatory effect of Zigui lip balm in vitro and in vivo
Objective:To evaluate the anti-inflammatory effects of Zigui lip balm both in vitro and in vivo.Methods:The model of inflammation was established using lipopolysaccharide-induced RAW 264.7 cells to detect the impact of Zigui alcohol extract on nitric oxide production.The model of atopic dermatitis-like mice induced by capsaicin was established to study the effects of Zigui lip balm on ear skin thickness,severity of ear skin inflammation,histopathology,and safety.Results:Zigui alcohol extract at 25-200 μg/mL could significantly promote the growth of RAW 264.7 cells and reduce nitric oxide levels.Meanwhile,Zigui lip balm could also significantly reduce ear thickness,eczema area and severity index(EASI)scores,inflammatory cell infiltration and decrease epidermal thickening and improve inflammation of the atopic dermatitis-like mice ear skin and in addition,Zigui lip balm had no effect on the liver,spleen,and kidneys of mice with capsaicin-induced atopic dermatitis.Conclusion:Zigui lip balm exhibits significant anti-inflammatory effects and is safe for use.
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