复方中药提取物冻干精华液对头皮组织衰老的影响
The effect of lyophilized essence of compound traditional Chinese medicine extracts on scalp tissue aging
梁晓进 1陈宪策 2倪春雅 3杨凯 3马彦云 4吴雪峰 5林尽染 1吴文育 6刘庆梅1
作者信息
- 1. 复旦大学附属华山医院皮肤科/上海市皮肤病研究所 上海 200040
- 2. 复旦大学生命科学学院 上海 200438
- 3. 上海市静安区中心医院皮肤科 上海 200040
- 4. 复旦大学人类表型组研究院 上海 200438
- 5. 贝拉医疗科技(苏州)有限公司 苏州 215300
- 6. 复旦大学附属华山医院皮肤科/上海市皮肤病研究所 上海 200040;上海市静安区中心医院皮肤科 上海 200040
- 折叠
摘要
目的:探究复方中药提取物冻干精华液对头皮组织衰老的影响.方法:以体外培养的正常人体头皮组织作为实验对象,并分为对照组和精华液处理组.采用William完全培养基进行组织培养,然后通过酶联免疫吸附试验、免疫荧光检测等技术,评估精华液对头皮组织衰老相关关键蛋白的影响.结果:在体外培养的头皮组织中,精华液在x/5、x/3、x/2 浓度下均能将白介素-6 表达水平降至基线的 1%以下.此外,精华液在x/10 浓度下可分别降低 25%的 8-羟基-2'-脱氧鸟苷(8-hydroxy-2'-deoxyguanosine,8-OHdG)表达、48%的硫酸软骨素蛋白多糖versican表达和 19%的髓鞘蛋白零样蛋白 3(myelin protein zero-like 3,MPZL3)表达(均P＜0.01),在x/5浓度下可分别降低 24%的 8-OHdG表达、24%的versican表达和 33%的MPZL3 表达(均P＜0.01).结论:复方中药提取物冻干精华液对体外培养的头皮组织具有抗炎、抗氧化和抗衰老的作用,具有开展进一步临床研究的价值.
Abstract
Objective:To explore the effects of lyophilized essence of compound traditional Chinese medicine extracts on scalp tissue aging.Methods:Normal human scalp tissues cultured in vitro were selected as the experimental subjects,which were divided into a control group and an extracts essence treatment group.The tissues were cultured in William's complete medium,and the effects of the extracts essence on the key proteins associated with scalp aging were evaluated by enzyme-linked immunosorbent assay and immunofluorescence detection.Results:In scalp tissues cultured in vitro,the extracts essence could significantly reduce the expression level of interleukin 6 to below 1%of the baseline at the concentrations of x/5,x/3,and x/2.In addition,the extracts essence could decrease the expression levels of 8-hydroxy-2'-deoxyguanosine(8-OHdG)by 25%,versican by 48%and myelin protein zero-like 3(MPZL3)by 19%at a concentration of x/10(all P<0.01),and the expression of 8-OHdG by 24%,versican by 24%and MPZL3 by 33%at a concentration of x/5(all P<0.01),respectively.Conclusion:The lyophilized essence of compound Chinese medicine extracts has anti-inflammatory,antioxidant and anti-aging effects on human scalp tissues cultured in vitro and a value of further clinical research.
关键词
复方中药提取物冻干精华液/头皮组织/抗衰老Key words
lyophilized essence of compound Chinese medicine extracts/scalp tissue/anti-aging引用本文复制引用
基金项目
上海市卫生健康领军人才项目(2022LJ017)
上海市静安区临床优势专病建设项目(2021ZB01)
上海毛发医学工程技术研究中心项目(19DZ2250500)
上海申康医院发展中心资助项目(SHDC22022302)
出版年
2024
NETL
NSTL
万方数据