Compositional analysis and antioxidant activity study of highland barley seed extracts
Objective:To compare the extraction process of highland barley seeds to maximize their antioxidant activities.Methods:Three methods,namely,biofermentation,aqueous extraction and alcohol extraction,were used to comparatively analyze the extraction efficiency of β-glucan and total phenols from barley seed grain extract.The antioxidant capacity of the three barley seed extracts was detected by ferric reducing antioxidant power(FRAP),2,2'-binamine-di-3-ethylbenzothiazolin-6-sulfonic acid(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging assay.Meanwhile,the identification of metabolite from the extracts was performed using high performance liquid chromatography with tandem mass spectrometry(LC-MS/MS).Results:The alcoholic extraction method showed optimal extraction,yielding 2.03 µg/mL β-glucan and 8.88 µg/mL gallic acid equivalent of total phenols,and the strongest antioxidant activity in all the three antioxidant activity assays,FRAP,ABTS,and DPPH.Twenty-five compounds were screened out by metabolite analysis,23 of which possessed antioxidant properties.Conclusion:This study reveals the metabolic composition of highland barley seed extract and its antioxidant activity,which provides a scientific basis for an in-depth understanding of the nutritional properties of barley and its potential health benefits,as well as theoretical support for the application of barley in the development of pharmaceuticals and nutraceuticals.
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