Molecular characteristics of human skin basement membrane barrier damaged by ultraviolet B(UVB)
Objective:To observe the characteristics of basement membrane barrier damage caused by UVB irradiation in cell models and isolated skin tissues.Methods:HaCaT cells were irradiated with 40 mJ/cm2 UVB,and the expression characteristics of basement membrane barrier-related genes were analyzed by RNA-seq data.In addition,transmission electron microscopy and immunofluorescence staining were used to observe the changes of basement membrane structure and key barrier protein content in three groups of isolated skin tissues after continuous irradiation with 50 mJ/cm2 UVB for 3 days.Results:Eighteen genes related to basement membrane were found,of which the expression of 17 genes were down-regulated.The expression levels of COL4A4,COL4A5,COL4A6,ITGB1,ITGA3,ITGA4,ITGA6 and LAMA5 was down-regulated by more than 1.5 times(P<0.05),while the expression level of HPSE was up-regulated by 2.4 times(P<0.000 1).In the in vitro skin tissue experiment,the transparent layer and dense layer of the basement membrane were observed to be broken after UVB irradiation,the hemidesmosomes were destroyed and their integrity and continuity were damaged,and the integrin protein content was decreased(P<0.05).Conclusion:UVB irradiation can down-regulate the expression of genes related to the structure of the basement membrane barrier,up-regulate the expression of heparanase gene,cause the disorder of the basement membrane structure and the degradation of the key barrier protein of the basement membrane,and damage the basement membrane.
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