Objective:The possible targets of ginsenoside Rb1 against cerebral ischemia-reperfusion injury were investigated by network pharmacology and molecular docking technology to explore the molecular mechanism of ginsenoside Rb1,so as to provide a theoretical basis for future experimental studies.Methods:Ginsenoside Rb1-related target genes were obtained from the SuperPred database,and abnormally expressed molecules in cerebral ischemia-reperfusion injury were obtained from the GEO,GeneCards and OMIM databases,and the possible targets of ginsenoside Rb1 acting on cerebral ischemia-reperfusion injury were obtained through intersection analysis.Effective genes and signaling pathways were enriched by the protein-protein interaction network,GO function enrichment analysis and KEGG signaling pathway enrichment analysis.AutoDock software was used to molecularly dock key targets and active ingredients to select the best binding target.Results:One hundred and sixty-three potential targets of ginsenoside Rb1 were obtained from SuperPred database,and 228 potential targets of cerebral ischemia-reperfusion injury from GeneCards and OMIM databases.A total of 14 targets of ginsenoside Rb1 were obtained after intersecting with cerebral ischemia-reperfusion injury.One hundred and fifty entries were obtained by GO functional enrichment analysis,and KEGG pathway enrichment analysis yielded 24 signaling pathways.The analysis of docking showed that ginsenoside Rb1 had stronger affinity with NFKB1 and STAT3.Conclusion:Ginsenoside Rb1 may exert anti-cerebral ischemia-reperfusion injury effects through NFKB1 and STAT3 signaling pathways.
NETL
NSTL
万方数据
