Study on the mechanism and specificity of an oncolytic adenovirus(recombinant human adenovirus type 5)inhibiting the proliferation of tumor cells
The mechanism and specificity of human adenovirus type 5(H101)in inhibiting tumor cells were studied.By in vitro cell culturation and TCID50 virus titer test,it was found that the oncolytic adenovirus had a significant selective replication effect on p53-tumor cells and its replication ability could produce oncolytic effect on p53 mutated tumor tissue and achieved therapeutic effect.In vitro pharmacodynamic experiment was used to detect the inhibitory effect of H101 on ten tumor cell lines.By calculating IC50 and IC90,it was further concluded that H101 was sensitive to the mouse hepatoma cell Hepa1-6,the mouse pancreatic cancer cell Pan02 and the mouse glioma cell GL261.These results showed that H101 had oncolytic effects on other tumor cells beyond the approved indications and a potential for expanding indications.This study can also provide reference for the selection of animal models and dosage for subsequent in vivo pharmacological experiments.
oncolytic adenovirustumor treatmentpharmacodynamic experiments in vitroproliferation inhibition
万方数据
维普
