Objective:To establish a rapid,convenient and low-cost evaluation method for the production process of biological enzyme preparations.Methods:Combined with gel electrophoresis and BCA protein quantitative method,more accurate quantitative data of target protein were obtained.Results:Taking the trypsin production process as an example,SDS-PAGE and BCA tests were performed on samples from different sections,and the corresponding protein quantitative data were obtained by calculation.The RSD≤5%of the sample data in the group indicated the repeatability of the method.Conclusion:This method can accurately analyze the changes of protein concentration in different sections and quickly and sensitively quantify the protein.It is found that there is a defect of protein leakage in original production process of trypsin,which provides a theoretical basis for subsequent process improvement.
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