Objective:To investigate the effects of different storage time,temperature and pretreatment methods on the results of blood species identification.Methods:The effects of storage time(one or three weeks)and temperature(4 or-20℃)and pretreatment methods(removing red blood cells or not)on species identification were investigated taking the blood of pig,bovine and sheep for samples.The samples were subjected to DNA extraction and PCR-RFLP detection for result determination.Results&Conclusion:The samples could be used for normal species identification when stored for one week and at 4 or-20℃and pretreated by removing red blood cells or not.The false-negative verdicts were generated when samples were stored for 3 weeks at either 4 or-20℃and the red blood cells were not removed during pretreatment,which may be due to the presence of PCR inhibitors in the extracted DNA.The species identification could be normally performed by removing red blood cells during pretreatment.This conclusion has general applicability to pig,bovine and sheep blood.
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