摘要
目的:社区获得性肺炎(CAP)是儿童住院和死亡的主要原因,本研究旨在从CAP中筛选免疫相关的基因.方法:从GEO数据库获取CAP数据,利用生物信息学方法筛选免疫相关基因,并进一步探究这些基因的功能及其与免疫细胞的关系.结果:CAP疾病组与正常组之间的差异基因主要与抗原加工和递呈、核糖体、细胞凋亡和炎症反应等功能相关.WGCNA和PPI分析共确定了 10 个免疫相关的关键基因:IL4R、FGR、CCR3、CCR1、ARG1、CEBPB、IL17RA、NFKBIA、PPARG和NCF4.这些基因的表达水平与免疫细胞的浸润水平呈显著的相关性.结论:本研究筛选出了可能在CAP免疫反应中起重要作用的基因.
Abstract
Objectives:Community-acquired pneumonia(CAP)is a leading cause of hospitalization and death in children.The present study mainly aimed to screen out immune-related genes in CAP.Methods:CAP data were retrieved from GEO online database.The immune-related genes were identified using bioinformatics method,and their function and relationship with immune cells were further investigated.Results:The differential expressed genes between CAP group and normal group were mainly related to antigen processing and presentation,ribosome,apoptosis and inflammatory response.Moreover,a total of 10 key immune-related genes were selected using WGCNA and PPI analysis,including IL4R,FGR,CCR3,CCR1,ARG1,CEBPB,IL17RA,NFKBIA,PPARG,and NCF4.The expression levels of these genes were significantly correlated with the infiltration level of immune cells.Conclusion:The key genes identified from this study might play important roles in the immune response of CAP.
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