Identification of WD40 gene family in pigeon pea and their expression pattern responded to Methyl Jasmonate
WD40 protein is mainly involved in the regulation of biological processes such as the growth and development of plant,syn-thesis of secondary metabolites and stress responses.WD40,as one of the MYB-bHLH-WD40(MBW)protein complexes,activates the transcription of genes which are located downstream of synthesis to promote anthocyanin accumulation.Exploring the function of WD40 gene family is the key procedure to analyze the metabolic regulation mechanism of flavonoid secondary metabolites.In this stud-y,based on the pigeon pea(Cajanus cajan)genome,the members of CcWD40 family genes were identified in pigeon pea,and the Cc-WD40 genes and its responses of Methyl Jasmonate were analyzed by bioinformatics and experiments respectively.With bioinformatics,116 members of the CcWD40 gene family were identified in the genome of pigeon pea,and the characteristics of the gene structure,chromosome distribution,cis-acting elements of the promoters,and the evolutionary history of the candidate genes were systematically and comprehensively evaluated,and the expression pattern disposed by Methyl Jasmonate was analyzed.The results showed that the a-mino acid residues of WD40 proteins ranged from 296 to 1 709,and the isoelectric point range was from 4.33 to 9.58;68 of the 116 genes could be mapped,and these 68 CcWD40 genes were unevenly distributed on 11 chromosomes,mostly located on chromosome 3;the phylogenetic tree divides the members of the pigeon pea CcWD40 family into 18 subfamilies.Although the introns of the CcWD40 gene structure differ greatly,they are relatively similar in the same branch of the evolutionary tree;collinearity analysis shows pigeon pea is the closest relative to soybean among these 3 model organisms;pigeon pea CcWD40 has multiple response elements,including stress response elements,developmental response elements and hormone response elements,such as abscisic acid response element(ABRE),salicylicacid response element(TCA-Element),gibberellin response element(GARE-Motif),etc.It can be seen that their expression is controlled by a complex regulatory network which may play an important role in abiotic stress;it is supposed that based on RNA-seq data analysis,the pigeon pea CcWD40 family genes significantly responded to the disposal of Methyl Jasmonate,in which 96 CcWD40 genes will express less while 20 ones express more and the CcWD40 genes located at the same branch of evolution share the similar responsive tendency.This study will provide important genetic resources and theoretical basis for further exploring the function of WD40 gene in regulating the synthesis of flavonoids and responding to abiotic stress.
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