Establishment and comparison of sensorineural deafness models in Mongolian gerbils
Objective Different ototoxic drugs were used to establish the model of sensorineural deafness in Mongolian gerbils,and the three models were compared to provide accurate animal models for the study of sensorineural deafness.Methods Mongolian gerbils model of sensorineural deafness was established by kanamycin sulfate and furosemide(KM+Fur),neomycin(Neo)and Ouabain(Oua),ABR auditory function and cochlear tissue morphological test were examined to compare and analyze the damage characteristics of different ototoxic drugs.Results After KM+Fur modeling,compared with the control group(14.00±1.80),the hearing threshold of Mongolian gerbils in this group(71.00±5.26)was significantly increased,and the cochlear HCs,while SGNs were not significantly damaged.After Neo modeling,40 mmol·L-1 Neo only affected the hearing threshold(60.00±8.66)at high frequency 32 kHz,and had no obvious histological changes.n the 100 mmol·L-1 Neo group,the hearing thresholds of all frequencies were significantly in-creased,and the cochlear HCs and SGNs were severely damaged.After the modeling of Oua,1 mmol·L-1 Oua and 10 mmol·L-1 Oua could significantly increase the hearing thresholds of all frequencies in gerbils,but the damage ranges were different.10 mmol·L-1 Oua severely damaged HCs and SGNs,while 1 mmol·L-1 Oua only damaged cochlear SGNs.Conclusion KM+Fur can be used to establish sensorineural deafness model with specific damage of HCs in gerbils.40 mmol·L-1 Neo can establish sensorineural deafness model with high frequency hearing impairment.Using 100 mmol·L-1 Neo and 10 mmol·L-1 Oua,the sensorineural hearing loss model of gerbils with impaired hair cells and spiral ganglion neurons was established.Deafness model with cochlear spiral ganglion neurons specific im-pairment of gerbils cochlea with 1 mmol·L-1 Oua was established.
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