Study on the preventive and therapeutic effects of ethyl acetate extract from chicory on ulcerative colitis complicated with acute liver injury in Rats
Objective To study the protective effect of Cichorium root ethyl acetate extract(CGEA)on acute liver injury complicated with acute colitis in Rats.Methods According to 6 Rats in each group,36 SD Rats were randomly divided into Control group,Model group,sulfasalazine enteric-coated tablet positive control group(100 mg·kg-1)and CGEA high and low dose groups(150,100 mg·kg-1),Except the normal group,the other groups were given enema with 30%TNBS-40%ethanol solution(60 mg·kg-1)and subcu-taneous injection of 2 mL·kg-1 olive oil solution containing 40%CCl4 twice a week to prepare acute liver injury model combined with ul-cerative colitis model.After successful modeling,CGEA was prepared into water suspension.According to the high-dose group(150 mg·kg-1)and low-dose group(100 mg·kg-1).Fourteen days later,the rats were killed,and the serum and liver and spleen tissues were taken,and the liver and spleen index was calculated,and the serum related indexes of rats were detected.The pathological chan-ges of liver and intestine were observed by HE staining.Results The index of liver and spleen in model group was significantly higher than that in normal group(P<0.01).The levels of AST,ALT,AKP,γ-GT and MDA in serum were also higher than those in normal group(P<0.05 or P<0.01)and the level of SOD decreased(P<0.05).Liver tissue was destroyed and inflammatory cells infiltrated;Colon tissue structure is disordered,and microvilli are lost and inflammatory cells are infiltrated.Compared with the model group,the index of liver and spleen in the medication group decreased significantly(P<0.01).Serum levels of AST,ALT,AKP,γ-GT and MDA decreased significantly(P<0.05 or P<0.01).The damage degree of liver and intestine tissue and the infiltration of inflammatory cells were reduced in the treatment group.Conclusion CGEA can alleviate the degree of acute liver injury complicated with ulcerative colitis in rats and protect it.
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