Identification of the dominant thrips species and the response of detoxification enzymes to control insecticides in the main sunflower-producing regions of Xinjiang
In order to identify the dominant population of harmful thrips in the main producing areas of sunflowers in Xinjiang,and screen an effective insecticide for controlling harmful thrips,as well as investigate the effects of the insecticide on the activities of main detoxification enzymes in the thrips.The species and dominant populations of harmful thrips were identified by using morphological and molecular biological identification techniques in the main sunflower-producing areas of Xinjiang.The insecticides for controlling the dominant thrips species were screened by using the leaf tube method,and the changes of the main detoxification enzyme activity in the dominant species of harmful thrips under sublethal concentration were determined by microplate reader.The results showed that the pri-mary harmful thrips were Frankliniella intonsa,F.occidentalis and Thrips tabaci.Among which,the F.intonsa was the dominant pop-ulation,accounting for 55.33%and 64.67%in the ninth and tenth divisions of Xinjiang,respectively.Among the four selected insec-ticides,spinetoram was exhibited the highest toxicity to the F.intonsa with LC50 of 0.021 mg·L-1.After treatment with LC25 dose of spinetoram for 48 h,the activity of AChE was decreased,while the activities of CYP450,AChE,MFOs and CarE were increased.There are three main species of harmful thrips endangering the main producing areas of sunflower in Xinjiang,in which the F.intonsa is the dominant population.The spinetoram has strong toxicity to F.intonsa and can affect the activity of the main detoxifying enzymes at sublethal concentration.Our study provides a theoretical basis for the rational use of insecticides to control the harmful thrips in sun-flower.
维普
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