Correlation analysis of IL18RAP expression with prognosis and CD8+T cell infiltration in patients with liver cancer
Objective To investigate the correlation between the IL18RAP gene and the prognosis of liver cancer patients and CD8+T cell infiltration in tumor microenvironment,and the possibility of IL18RAP as a tumor biomarker.Methods The Cancer Genome Atlas(TCGA)was used to evaluate the expression of IL18RAP in liver cancer.Univariate Cox analysis,multivariate COX analysis,and sur-vival analysis revealed the prognostic value of the IL18RAP.KEGG,GO,and Hallmark enrichment analyses were conducted to identify functional pathways associated with the IL18RAP.Immune infiltration analysis was conducted to explore the relationship between the IL18RAP gene and 22 types of immune cell infiltration.The correlation between IL18RAP and CD8+T cell infiltration was verified by single-cell sequencing database and immunohistochemistry.Results IL18RAP is downregulated in liver cancer tissues and its low ex-pression is associated with poor prognosis in patients with liver cancer.Functional enrichment analysis showed that the expression of IL18RAP is related to immune function pathways.Immune infiltration,single-cell sequencing,and immunohistochemistry showed that IL18RAP was highly expressed in CD8+T cells.Conclusion Low expression of IL18RAP is associated with poor prognosis in liver cancer patients,which may affect the anti-tumor immune of CD8+T cells.
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