Objective To investigate the role of SUMO modification of SP3 in macrophages infected with Echinococcus granulosus.Methods In vivo,the mice were randomly divided into control group(PBS)and experimental group(subcapsular suspension of the o-riginal head segment was inoculated).The expressions of SUMO1 and UBC9 in liver were detected by immunohistochemistry.Fluores-cence co-localization of macrophage CD206 and SUMO1 was detected by immunofluorescence double staining.RAW264.7 macrophages were divided into control group,LPS group and LPS+EgCF group.qRTPCR was collected at 12 h to detect the mRNA expression of SUMO1,UBC9,IL-6 and IL-10.The protein levels of SUMO1 and UBC9 were detected by Western blot.COIP detects the interac-tion between SUMO1 and Sp3.Results In vivo experiment:Compared with the control group,SUMO1 and UBC9 proteins were highly expressed in the inflammatory area of liver tissue near the cyst in experimental group,and CD206+macrophages were co-localized with SUMO1.In vitro experiments:EgCF stimulated the inflammatory model of RAW264.7 cells to promote IL-10 expression,inhibit IL-6 expression,induce SUMO1,UBC9 mRNA and protein expression,and induce SUMO of Sp3.Conclusion Echinococcus granulosus infection induces SUMO modification of macrophage Sp3.
维普
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