The SUMO modification of macrophage Sp3 induced by Echinococcus granulosus infection
Objective To investigate the role of SUMO modification of SP3 in macrophages infected with Echinococcus granulosus.Methods In vivo,the mice were randomly divided into control group(PBS)and experimental group(subcapsular suspension of the o-riginal head segment was inoculated).The expressions of SUMO1 and UBC9 in liver were detected by immunohistochemistry.Fluores-cence co-localization of macrophage CD206 and SUMO1 was detected by immunofluorescence double staining.RAW264.7 macrophages were divided into control group,LPS group and LPS+EgCF group.qRTPCR was collected at 12 h to detect the mRNA expression of SUMO1,UBC9,IL-6 and IL-10.The protein levels of SUMO1 and UBC9 were detected by Western blot.COIP detects the interac-tion between SUMO1 and Sp3.Results In vivo experiment:Compared with the control group,SUMO1 and UBC9 proteins were highly expressed in the inflammatory area of liver tissue near the cyst in experimental group,and CD206+macrophages were co-localized with SUMO1.In vitro experiments:EgCF stimulated the inflammatory model of RAW264.7 cells to promote IL-10 expression,inhibit IL-6 expression,induce SUMO1,UBC9 mRNA and protein expression,and induce SUMO of Sp3.Conclusion Echinococcus granulosus infection induces SUMO modification of macrophage Sp3.
万方数据
维普
