The evaluation of the anti-heat stroke effect of Rehmannia glutinosa based on the inflammatory factor network
Objective Heat stroke(HS)is a complex disease caused by acute heat injury in high-temperature and high-humidity envi-ronments.Its occurrence and development are closely related to a variety of inflammatory factors in the blood.The therapeutical effects of heat-clearing and blood-cooling medicine Rehmannia glutinosa(RG)for heat stroke is still unclear.Methods The literature on'heat stroke'was retrieved from CNKI and Pubmed.Under the guidance of statistics,the markers of blood inflammatory factors with signifi-cant changes in HS were manually excavated and a scale-free network was constructed.The RG's active components were collected,and the related targets were predicted and correlated with the heat stroke blood inflammatory factor network.The heat stroke mouse model in the environment of temperature(41±0.5)℃and relative humidity 60%±5%was made in the artificial climate chamber to e-valuate the anti-HS efficacy of the crude extract of RG.The related inflammatory factors in the tissues were detected by qRT-PCR,and the drug-target association was verified by molecular docking.Results The related biological processes and pathways between RG and heat stroke are mainly concentrated in an inflammatory response and apoptotic response.The results of biological verification showed that RG could prolong the survival time of heat stroke mice,improve the survival rate of mice,reduce acute reactions such as liver and kidney swelling,and significantly improve liver and kidney damage(P<0.05).The results of qRT-PCR showed that the crude extract of RG could significantly reduce the mRNA expression levels of inflammatory factors IL-6,IL-1β,IL-17 A,NF-κB and TNF-α(p<0.05),and play an anti-inflammatory role.These factors play an important role in the network of blood inflammatory factors in heat stroke.Molecular docking results showed that the active components had good binding activity with key proteins.Conclusion RG exerts its anti-heatstroke pharmacological effect by inhibiting inflammatory responses and improving liver and kidney damage.
万方数据
维普
