To screen and verify the differentially expressed genes in post-traumatic stress disorder based on bioinformatics
Objective To screen and verify the differentially expressed genes(DEG)in post-traumatic stress disorder(PTSD),and further explore the possible mechanism of PTSD.Method Peripheral blood mononuclear cells(PBMC)were collected from 26 PTSD patients and 26 healthy people,respectively.According to the experimental design and technical requirements,they were divided into gene screening group(n1=6)and gene validation group(n2=20).First,the new gene pool was established by RNA sequencing tech-nology(RNA-Seq),and then the DEG of PTSD patients were screened by GO analysis,KEGG analysis and other methods,and veri-fied by real-time fluorescent quantitative polymerase chain reaction(q-RT-PCR).Plasma samples were collected from participants,and the DEG was further validated by enzyme-linked immunosorbent assay(ELISA).Results(1)A total of 609 DEG was screened out,of which 448 genes were up-regulated and 161 genes were down-regulated.(2)Four genes were verified by q-RT-PCR,among which the expression of FPR1 and CD14 was significantly increased,and the difference was statistically significant(P＜0.05);The ex-pression of XCL2 and TIGIT decreased,but the difference was not statistically significant.(3)Four genes were verified by ELISA:CD14,FPR1 and XCL2 were down-regulated at the protein level,and the difference was statistically significant(P＜0.05);However,there was no significant difference in the protein expression of TIGIT,and it was not statistically significant(P＜0.05).Conclusion CD14 and FPR1 for PTSD occurred in the development of differentially expressed genes,it might by inflammatory pathways to influence the occurrence of PTSD development.However,the relationship between XCL2 and TIGIT and the development of PSTD remains to be explored.
万方数据
维普
