Effects of hydrolyzed tannin acid on intestinal health of layers
To study the effects of hydrolyzed tannin acid on intestinal pH,enzyme activity and microflora,4 000 30-week-old hyline layers were randomly divided into five groups with four replications,each replication per 200 chickens.Five kinds of diets were fed respectively for four weeks:base diet (control),basal diet + 0.05% hydrolyzed tannin acid,basal diet + 0.10% hydrolyzed tannin acid,basal diet + 0.15% hydrolyzed tannin acid,basal diet + 0.20% hydrolyzed tannin acid.The results showed that on intestine enzyme activity group Ⅱ had a significant effects on lipase activity and amylase activity of duodenum and jejunum (P<0.05).GroupⅢ had a significant effects on amylase activity of ileum (P<0.05).Group Ⅰ had a significant effects on protease activity of duodenum (P<0.05).Group Ⅱ and Ⅲ had a significant effects on protease activity of duodenum,jejunum and ileum (P<0.05).On intestinal microflora,hydrolyzed tannin acid could increase the beneficial bacterium,at the same time,inhibit the beneficial bacterium,group Ⅱ and Ⅲ (P<0.05).Adding hydrolyzed tannin acid (1 kg/t) can decrease intestinal pH,improve the intestinal digestive enzyme activity and intestinal health of layers.