Analysis of microbial diversity and metabolic function difference in donkey between foregut and hindgut
The donkey's hindgut is a complex fermenter that plays a vital role in the donkey's health and energy supply. So far, there has been no report on the difference of intestinal microbial diversity in the foregut and hindgut of donkeys. In this study, the contents of the foregut and hindgut were col⁃lected after slaughter of jacks aged about 2 years old, and high-throughput sequencing was conducted in the v3-v4 region of 16S rRNA gene to characterize and predict the microbial flora of the intestinal tract of donkeys. The results showed that: ① A total of 3 106 234 clean data were obtained in this study, and 7 459 Operational Taxonomic Units (OTU) were obtained after species annotation, belong⁃ing to 35 phylum, 55 classes, 118 orders, 222 families and 512 genera. The number of core OUTs was 2 960, and 996 in the foregut and 2 059 in the hindgut were unique. ② The richness index of bacterial species, diversity index, phylogenetic diversity index were significantly different be⁃tween the foregut and hindgut of donkey (P<0.01). ③ The phylum Firmicutes was dominant in both foregut and hindgut, Firmicutes (92.4% ), Proteobacteria (3.5% ), Bacteroidetes (2.7% ) and Actinomyces (0.8% ) in foregut; and Firmicutes (55.7% ), Bacteroidetes (34.2% ), Spirochetes (3.7% ) and Proteobacteria (3.1%) in the hindgut.④The genus Lactobacillus was prevalent in all samples, Lactoba⁃cillus (50.1%), Streptococcus (21.2%), Clostridium (10.4%), Actinomyces (2.0%) in the foregut; and Lac⁃tobacillus (5.7%), Streptococcus (4.4%), Helicobacter (2.2%), Clostridium (1.1%) in the hindgut.⑤Us⁃ing FAPROTAX to predict the function found that functions in foregut were relatively simple function of nitrogen metabolism, mammal and human gut, fermentation and chemoheterotrophic were mainly concen⁃trated in the foregut, while the functions in the hindgut were mainly anaerobic and oxygenation, human infectious diseases. These results are consistent with the functional differences and characteristics be⁃tween foregut and hindgut of donkeys. This study lays a foundation for further understanding of the di⁃gestion and absorption mechanism of donkeys, which can formulate more appropriate feeding programs.
NETL
NSTL
万方数据
维普
