The experiment was conducted to investigate the effect of fermentation condition on the produc-tion of acid from maize flour fermented by Enterococcus faecus fermented.The single-factor method was used to screen the basic parameters,and the single-factor results were subjected to an orthogonal design with 3 levels of 4 factors(inoculum,material to water ratio,fermentation time,and fermentation tempera-ture).The condition parameters of Enterococcus faecus fermented corn flour were optimized by measuring the activity,pH,soluble sugar and volatile fatty acid of Enterococcus faecus in fermented corn flour.The results showed that the differences of pH value by inoculum levels of 5%,15%and 25%were significant(P＜0.05);the differences of pH value with feed to water ratios of 1∶0.8,1∶1.2 and 1∶1.4 were signifi-cant(P＜0.05);and the differences of pH value at fermentation times of 24 h,48 h and 72 h were signifi-cant(P＜0.05).The pH value at fermentation temperature of 37℃was significantly lower than that at 25℃and 30℃(P＜0.05).The optimal fermentation conditions parameters of Enterococcus faecus fer-mented maize flour were:inoculum amount 25%,material to water ratio 1∶1.4,fermentation time 24 h,fermentation temperature 30℃.
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