Effects of Ellagic Acid on Gut Microbiota and Lipid Metabolism of Weaned Piglets
The experiment aims to study the regulatory effects of ellagic acid on the function and composi-tion of ileal microorganisms in piglets with oxidative stress,and to further analyze the effects of intestinal microorganisms on intestinal nutrient absorption and metabolism in weaned piglets.Twenty-four 21-day-old weaned piglets with body weight of(7.42±0.10)kg were divided into four groups(6 piglets per group),PQ group(negative control group)fed with basal feed,antibiotic group(AMP+PQ,positive control group)fed with basal feed supplemented with 24.3 mg/kg BW AMP,EA group(EA+PQ)fed with basal feed supple-mented with 0.01%EA,EA+AMP group(EA+AMP+PQ)fed with basal feed supplemented with 0.01%EA and 24.3 mg/kg(BW)AMP.On the 14th and 18th days of the experiment,paraquat powder was dissolved in 0.9%sterile saline solution with a final dose of 4 mg/kg BW for intraperitoneal injection.The experiment lasted for 28 days(adaptation period was 7 days).The results indicated that compared with PQ group,ellagic acid intervention signifi-cantly increased the β-diversity of ileal microor-ganisms,promoted the enrichment of Lactobacillaceae and inhibited the amplification of Streptococcaceae at the family level.Transcriptome data showed that PPAR,a signaling pathway related to fat metabolism,was significantly activated in the ileum of piglets supplemented with ellagic acid,and mRNA expression of pathways related to fat digestion and absorption,cholesterol metabolism and arachidonic acid metabolism was also significantly increased.In addition,spearman analysis revealed that the abundance of ileum micro-organisms FCPU426 and SAR324_clade.Marine,which were related to fat absorption and metabolism,increased in the ellagic acid treatment group,while the abundance of Euryarchaeota,which were negatively related to lipid metabolism,decreased.The results revealed that dietary ellagic acid can regulate the intesti-nal microbial structure of piglets under stress,and increase the enrichment of beneficial bacteria such as Lactobacillus and microorganisms related to lipid metabolism.At the same time,ellagic acid can also increase the expression of genes related to fat metabolism in the ileum of piglets,so as to improve the absorp-tion and metabolism of lipids in weaned piglets.
万方数据
维普
