血根碱和蒲公英提取物对黄鳝生长性能、血清生化指标、抗氧化功能和肠道形态结构的影响
Effects of Sanguinarine and Dandelion Extract on Growth Performance,Blood Biochemical Indices,Antioxidant Function and Intestinal Morphology and Structure in Finless Eel
陈靖 1刘立鹤 1徐朋涛 1刘诚傲 1何来 1尚宇博 1于鹏远 1丁斌鹰1
作者信息
- 1. 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,动物营养与饲料科学湖北省重点实验室,湖北 武汉 430023
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摘要
研究旨在探讨饲料中添加血根碱和蒲公英提取物对黄鳝生长性能、血清生化指标、抗氧化功能和肠道形态结构的影响.试验共选择8 000尾质量为(20±0.1)g的黄鳝,分为4个处理组,每个处理5个重复,每个重复400尾.试验组饲喂基础日粮(CON组),试验组在基础饲料中分别添加1 g/kg血根碱(T1 组)、3 g/kg蒲公英提取物(T2 组)以及 1 g/kg血根碱与 3 g/kg蒲公英提取物(T3组).试验期共7周,预试期2周,正试期5周.结果显示,与CON组相比,各试验组的黄鳝增重率、饲料系数、特定生长率、体长增长率和肠道增长率均无显著变化(P>0.05);与CON组相比,各试验组黄鳝血清TG含量均显著降低(P<0.05),T1、T2组黄鳝血清BUN含量显著降低(P<0.05);与CON组相比,T1、T2组黄鳝肠道T-AOC和T-SOD活性显著降低(P<0.05),T3组的黄鳝肠道CAT活性显著低于其余各组(P<0.05);与CON组相比,T2组黄鳝小肠绒毛高度显著升高(P<0.05),T1组小肠隐窝深度显著低于T2和T3组(P<0.05),T1组黄鳝小肠绒毛高度/隐窝深度的比值显著高于CON组和T3组(P<0.05).综上所述,在饲粮中单独添加血根碱或蒲公英提取物可以改善肠道形态结构和生长性能,同时降低黄鳝血清中BUN与TG的含量,但未能提高黄鳝肠道抗氧化能力.
Abstract
The aim of this study was to investigate the effects of sanguinarine and dandelion extract on growth performance,blood biochemical indices,antioxidant function and intestinal morphology and struc-ture in finless eel.A total of 8 000 eels with mass of(20±0.1)g were selected for the experiment,which were divided into four treatment groups with five replicates of 400 eels each.The groups were as follows:basal diet group(CON group),basal diet group with 1g/kg sanguinarine(T1 group),3 g/kg dandelion extract(T2 group),and 1 g/kg sanguinarine with 3 g/kg dandelion extract(T3 group).The experimental period totaled 7 weeks,with a pre-feeding period of 2 weeks and an official period of 5 weeks.The results showed that,compared with CON group,all experimental groups had no significant effect on weight gain rate,bait coeffi-cient,specific growth rate,body length growth rate and gut growth rate of eels(P>0.05);compared with CON group,the serum TG content of all treat-ment groups was significantly reduced(P<0.05),and the serum BUN content of the T1 and T2 groups was significantly reduced(P<0.05).Com-pared with CON group,serum T-SOD activity and T-AOC was significantly lower in the T1 and T2 groups(P<0.05);CAT activity was significantly lower in the T3 group than other groups(P<0.05).Compared with CON group,small intestinal villus height was significantly higher in the T2 group(P<0.05),small intestinal crypt depth was significantly lower in the T1 group than in the T2 and T3 groups(P<0.05),and the ratio of villus height/crypt depth in the T1 group was significantly higher than CON and T3 groups(P<0.05).In con-clusion,the addition of sanguinarine and dandelion extract alone to the ration improves the intestinal mor-phology,structure and growth performance of eels.And it reduces the content of BUN and TG in the serum of eel,but fails to improve the antioxidant capacity of eel intestine.
关键词
黄鳝/生长性能/肠道健康/血根碱/蒲公英提取物Key words
finless eel/growth performance/gut health/sanguinarine/dandelion extract引用本文复制引用
出版年
2025