Immunoadjuvant Activity of GM-CSF and IL-6 and Its Effects on Growth Performance and Blood Indexes in Mice
In order to verify the immunoadjuvant activities of GM-CSF and IL-6 and study their effects on growth performance and blood indexes of mice,the GM-CSF gene and IL-6 gene were amplified in this experiment,and the GM-CSF gene and IL-6 gene were cloned into the vector pCDNA3.1-His-A and pET-32a(+),respectively.And induced expression of IL-6 protein.The recombinant vector of GM-CSF gene and the induced expression of IL-6 recombinant protein were combined with the p-N-His vector of TGEV DNA vaccine previously con-structed by the animal disease prevention and con-trol technology research and development group to immunize mice(p-N-His was set as the control),and the specific antibody level was detected by indirect ELISA.The constructed recombinant vector,the induced expression of recombinant protein and PBS were divided into groups to immunize mice,and the growth per-formance,serum index and immune organ index of mice were measured.The results showed that the cloned GM-CSF and IL-6 genes were 579 bp and 576 bp respectively,and the eukaryotic expression vector p-GM-CSF and the original expression vector pET-IL-6 were successfully constructed,and the induced expres-sion of IL-6 recombinant protein was 38 ku.After the carrier p-GM-CSF and IL-6 protein combined with p-N-His immunized mice,p-N-His+p-GM-CSF,p-N-His+IL-6 protein and p-N-His stimulated the body to produce specific antibodies.The level of p-N-His+IL-6 protein antibody was higher than that of p-N-His+p-GM-CSF(P<0.05).After immunizing mice with IL-6 protein,p-GM-CSF and PBS,the growth performance indexes of mice in p-GM-CSF and IL-6 protein groups were higher than those in PBS group,and there were significant differences in the final weight,average daily gain and ratio of feed to gain(P<0.05).There were no significant differences in the contents of total protein,albumin,globulin and urea nitro-gen among the p-GM-CSF,IL-6 protein and PBS groups(P>0.05),but the glucose of the IL-6 protein group was significantly increased(P<0.05).There were no significant differences in immune organ index of p-GM-CSF,IL-6 protein and PBS groups(P>0.05).In conclusion,we used p-N-His as the target vector to verify that GM-CSF and IL-6 have strong immune adjuvant activity,and the effect of IL-6 protein is stron-ger than that of GM-CSF.p-GM-CSF and IL-6 protein can improve the growth performance index of mice,but have little effect on blood index and immune organ index.
万方数据
维普
