Research on Solid-State Fermentation Process of Full-Price Feed for Kunming Dogs
The purpose of this study was to optimize the process conditions of solid-state fermentation for full puppy food with Lactobacillus reuteri and Lactobacillus gasseri,and to explore the changes of nutrient and enzyme activity before and after fermentation.Using a single factor experimental design,the optimal fer-mentation temperature,solid-liquid ratio,fermentation time,inoculation amount and inoculation ratio for compound lactic acid bacteria fermentation were selected based on the number of lactic acid bacteria colo-nies in the fermentation products,and the optimal fermentation conditions were explored by response sur-face method.The results showed that the optimal fermentation process of compound lactic acid bacteria were as follows:the inoculation amount of compound bacteria was 1.8×107 CFU/g,the inoculation ratio of com-pound bacteria was 1∶1.0,the monoglyceride addition amount was 200 mg/kg,the solid-liquid ratio was 1∶1.2 g/mL,the fermentation temperature was 35℃,and the fermentation time was 36 h.After fermen-tation by Lactobacillus reuteri and Lactobacillus gasseri,the contents of crude protein and crude fat in the fer-mentation products increased significantly(P<0.05).However,the crude ash,calcium and total phosphorus did not change significantly(P>0.05).It could be concluded that the solid-state fermentation of Lactobacil-lus reuteri and Lactobacillus gasseri can improve the feeding value of Kunming dog food.