昆明犬幼犬全价饲料固态发酵工艺研究
Research on Solid-State Fermentation Process of Full-Price Feed for Kunming Dogs
胡敏洁 1展丽梅 1李吉琴 1自绍平 1高彦航 1徐乐 1李杰 2曹振辉1
作者信息
- 1. 云南农业大学动物科学技术学院,云南 昆明 650201
- 2. 公安部昆明警犬基地,云南 昆明 650201
- 折叠
摘要
试验旨在对罗伊氏乳杆菌和格氏乳杆菌固态发酵全价幼犬粮进行工艺条件优化,并对其发酵前后营养物质和酶活变化进行研究.采用单因素试验设计,以发酵产物中乳酸菌菌落数为指标,筛选复合乳酸菌发酵适宜的发酵温度、料液比、发酵时间、接种量、接种比例,并通过响应面法探究最佳发酵条件.结果表明:复合乳酸菌最优发酵工艺为复合菌接种量1.8×107 CFU/g,复合菌接种比例1∶1.0,单甘酯添加量200 mg/kg,料液比1∶1.2 g/mL,发酵温度35℃,发酵时间36 h.经罗伊氏乳杆菌和格氏乳杆菌发酵后,发酵产物中粗蛋白和粗脂肪含量显著提高(P<0.05),粗灰分、钙和总磷含量无显著变化(P>0.05).由此可见,罗伊氏乳杆菌和格氏乳杆菌固态发酵可提高昆明犬幼犬粮的饲用价值.
Abstract
The purpose of this study was to optimize the process conditions of solid-state fermentation for full puppy food with Lactobacillus reuteri and Lactobacillus gasseri,and to explore the changes of nutrient and enzyme activity before and after fermentation.Using a single factor experimental design,the optimal fer-mentation temperature,solid-liquid ratio,fermentation time,inoculation amount and inoculation ratio for compound lactic acid bacteria fermentation were selected based on the number of lactic acid bacteria colo-nies in the fermentation products,and the optimal fermentation conditions were explored by response sur-face method.The results showed that the optimal fermentation process of compound lactic acid bacteria were as follows:the inoculation amount of compound bacteria was 1.8×107 CFU/g,the inoculation ratio of com-pound bacteria was 1∶1.0,the monoglyceride addition amount was 200 mg/kg,the solid-liquid ratio was 1∶1.2 g/mL,the fermentation temperature was 35℃,and the fermentation time was 36 h.After fermen-tation by Lactobacillus reuteri and Lactobacillus gasseri,the contents of crude protein and crude fat in the fer-mentation products increased significantly(P<0.05).However,the crude ash,calcium and total phosphorus did not change significantly(P>0.05).It could be concluded that the solid-state fermentation of Lactobacil-lus reuteri and Lactobacillus gasseri can improve the feeding value of Kunming dog food.
关键词
乳菌酸/昆明犬/固态发酵/响应面优化/营养成分Key words
Lactobacillus/Kunming dog/solid-state fermentation/response surface optimization/nutrition facts引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据