石香薷精油对小鼠肠道炎症和微生物屏障的影响
Effect of Essential Oil of Mosla chinensis Maxim.on Intestinal Inflammation and Microbial Barrier in Mice
师盼盼 1李平萍 1苏越 2黄嘉璐 2付玥 1谭桂凤 1黄鹏3
作者信息
- 1. 湖南农业大学动物科学技术学院,湖南 长沙 410000
- 2. 山西农业大学动物医学院,山西 晋中 030600
- 3. 湖南农业大学动物科学技术学院,湖南 长沙 410000;湖南农业大学中兽药湖南省重点实验室,湖南 长沙 410000
- 折叠
摘要
试验旨在研究石香薷精油对小鼠肠道炎症和微生物屏障的影响.试验将90只小鼠平均分为6组,每组15只,试验期7 d.空白组(B组)和模型组(MC组)给小鼠灌胃生理盐水,阳性对照组(P组)灌胃盐酸小檗碱,石香薷精油组则通过灌胃给小鼠饲喂不同浓度的石香薷精油[浓度分别为0.062 5(LD组)、0.250 0(MD组)、0.500 0 mL/kg(HD组)的石香薷精油].最后1 d,饥饿16 h后,除空白组(B组)外,所有小鼠腹腔注射浓度为6 mg/kg的脂多糖(LPS).同时,将每组的5只小鼠放入代谢笼中2 h,通过测量滤纸对腹泻情况进行评估.随然,实时荧光定量PCR分析了各组组织中炎症基因的表达情况.此外,还分析评估了石香薷精油对肠道微生物菌群的影响.研究结果表明,石香薷精油不仅能减轻腹泻和肠道炎症,还能改善肠道菌群的组成.因此石香薷精油具有一定的降低炎症与改善肠道微生物屏障的能力.
Abstract
The aim of this study was to investigate the effects on intestinal inflammation and microbial bar-rier of essential oil of Mosla chinensis Maxim..Ninety mice were divided into 6 groups,with 15 mice in each group.During the next seven days,the blank group(group B)and model group(group MC)were orally administered normal saline,the positive control group(group P)was orally administered berberine hydro-chloride,and the group of essential oil of Mosla chinensis Maxim.was orally administered essential oil of Mosla chinensis Maxim.at different concentrations[0.062 5(LD),0.250 0(MD),0.500 0(HD)mL/kg].On the final day,after a 16-hour fast,all mice except those in the blank group were intraperitoneally injected with LPS at a concentration of 6 mg/kg.At the same time,five mice from each group were placed in metabolic cages for two hours to evaluate diarrhea using filter paper.Subsequently,the study analysed the expression of inflammatory genes in each group of tissues using quantitative real-time PCR(qPCR).In addition,this study also analysed and assessed the effect of essential oil of Mosla chinensis Maxim.on the gut microbiota.These findings suggested that essential oil of Mosla chinensis Maxim.not only reduces the severity of diarrhea and intestinal inflammation,but also improves the composition of the gut microbiota.Therefore essential oil of Mosla chi-nensis Maxim.has the ability to reduce inflamma-tion and improve the intestinal microbial barrier to some extent.
关键词
石香薷精油/肠道菌群/炎症/腹泻/肠道微生物屏障Key words
essential oil of Mosla chinensis Maxim./intestinal microbiota/inflammation/diarrhea/intestinal microbial barrier引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据