糖尿病诱导的小鼠室旁核催产素和加压素阳性神经元FOS表达
FOS expression in oxytocin and vasopressin positive neurons in paraventricular nucleus of mice induced by diabetes
王淑敏 1贾鹏 2任淑婷 3吕思婷 4张婷 5杨彦玲 6史娟5
作者信息
- 1. 延安大学医学院生理学教研室,延安 716000;空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032
- 2. 中国人民解放军94750部队医院,龙岩 366200
- 3. 空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032;延安大学医学院解剖学教研室,延安 716000
- 4. 空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032;延安大学医学院病原生物学教研室,延安 716000
- 5. 空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安 710032
- 6. 延安大学医学院生理学教研室,延安 716000
- 折叠
摘要
目的:探索糖尿病(DM)不同状态下小鼠下丘脑室旁核(PVN)催产素和加压素阳性神经元的FOS表达变化.方法:腹腔注射溶媒或链脲佐菌素(STZ)制备对照组或糖尿病小鼠模型,根据机械痛测试区分糖尿病痛(DNP组)与非痛组(DWP组).采用免疫组化和免疫荧光染色技术检测PVN内FOS、催产素(OXT)和加压素(VP)阳性神经元的分布及双标情况,并进行计数和比较.结果:7 d时三组(Control组、DNP组和DWP组)小鼠PVN内均有较多的FOS表达,而28 d时DWP和DNP组FOS的表达几近于无,与7 d组相比差异显著(P＜0.05或0.001).与对照组相比,DNP组和DWP组动物的VP和OXT荧光染色同样存在随着造模时间延长染色强度减弱的趋势(P＜0.05).VP、OXT与FOS的双标染色计数结果显示,在DWP 7 d组,VP/FOS双标细胞数为74.33±22.10,占VP阳性细胞数的(56.64±7.52)％,而OXT/FOS的双标率则仅为(10.44±3.14)％.在DNP7d组,OXT/FOS双标细胞数为51.00±31.80,占OXT阳性细胞数的(18.50±9.51)％,而VP/FOS的双标率仅为(9.34±3.27)％.与此相对,28 d组FOS的表达锐减,几乎无双标细胞.结论:下丘脑PVN内的VP和OXT阳性神经元在糖尿病痛与非痛,疾病发展的不同阶段存在明显不同的可塑性变化特点,理解这些变化对于揭示糖尿病及其并发症的神经机制具有重要意义.
Abstract
Objective:To explore the feature of FOS expression in oxytocin-and vasopressin-positive neurons in the hypothalamic paraventricular nucleus(PVN)under different status of diabetes mellitus(DM).Methods:Intraperito-neal injection of vehicle or STZ in mice was conducted to establish control or diabetes model.Mechanical sensitivity was evaluated by von Frey filament tests to distinguish diabetic neuropathic pain(DNP)from without-pain group(DWP).The expression of FOS,oxytocin(OXT)-and vasopressin(VP)-positive neurons,as well as their double labeling was detected by immunohistochemical and immunofluorescent staining.Cell counting and comparison were made in groups.Results:FOS expression was easily detected in the PVN in the three groups(Control group,DNP group and DWP group)at 7 days,while that in DWP and DNP groups at 28 days was hardly detectable,with the number being signifi-cantly different from the 7 days group(P＜0.05 or 0.001).Likewise,compared with the control group,immunofluo-rescent signals for VP and OXT staining in the DNP and DWP groups also showed a trend of weakening as the modeling time increased(P＜0.05).The cell counting after double staining for VP or OXT with FOS showed that,in the DWP group at 7 days,the number of VP and FOS double-labeled neurons was 74.33±22.10,accounting for(56.64± 7.52)％of VP-positive cells,whereas the double labeling rate for OXT and FOS was only(10.44±3.14)％.In the DNP group at 7 days,the number of OXT and FOS double-labeled neurons was 51.00±31.80,accounting for(18.50 ±9.51)％of OXT-positive neurons,whereas the double labeling rate for VP and FOS was only(9.34±3.27)％.In contrast to these changes in 7 days group,the expression of FOS decreased sharply in the group of 28 days,thereby al-most no double-labeled neurons.Conclusion:The plasticity changes of oxytocin-and vasopressin-positive neurons in the PVN are different depending on the status of pain and non-pain,and the stage of disease progression.Understanding the changes is of great significance for unravelling the neural mechanism of diabetes and its complications.
关键词
糖尿病神经病理性痛/下丘脑室旁核/催产素/加压素/小鼠Key words
diabetic neuropathic pain(DNP)/hypothalamic paraventricular nucleus(PVN)/oxytocin(OXT)/vasopressin(VP)/mouse引用本文复制引用
基金项目
国家自然科学基金(82071253)
国家自然科学基金(31571188)
出版年
2024
NETL
NSTL
万方数据