天麻素对缺血缺氧诱导的小胶质细胞中TLR4表达的影响
The effects of gastrodin on TLR4 expression in microglia induced by ischemia and hypoxia
李顺达 1赵永强 1杨雪珂 2陈澍雨 3杨艳 4袁云1
作者信息
- 1. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500
- 2. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500;三峡大学第一临床医学院输血科,宜昌 443003
- 3. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500;江西省妇幼保健院辅助生殖中心,南昌 330006
- 4. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500;四川携光生物技术有限公司,成都 611731
- 折叠
摘要
目的:探究天麻素(GAS)对缺血缺氧性损伤(HIBD)后小胶质细胞toll样受体4(TLR4)表达的影响.方法:体内创建新生大鼠HIBD模型,将30只3 d龄SD大鼠随机组成3组:假手术组(sham)、HIBD模型组、HIBD模型+GAS干预组(HIBD+G);体外创建BV2细胞氧糖剥夺(OGD)模型,实验随机设置对照组(control)、OGD组、OGD+GAS干预组(OGD+G).Western Blot和免疫荧光双标染色技术均检测体外各组细胞及体内大鼠模型左侧大脑胼胝体区TLR4的表达.结果:OGD诱导的小胶质细胞中TLR4表达显著增加;GAS干预后TLR4表达显著减少(P＜0.05).结论:GAS激活的小胶质细胞TLR4的表达具有抑制效应,从而对HIBD发挥神经保护作用.
Abstract
Objective:To determine the effect of gastrodin(GAS)on toll-like receptor 4(TLR4)expression in mi-croglia after hypoxic-ischemic brain damage.Methods:Hypoxia-ischemic brain damage(HIBD)model was established in neonatal rat in vivo.Thirty 3 d SD rats of were randomly divided into there groups:Sham group,HIBD model group,HIBD model+gastrodin intervention group(HIBD+G).Oxygen glucose deprivation(OGD)model was established in BV2 cells in vitro,Control group(Control),oxygen glucose deprivation group(OGD),OGD+gastrodin intervention group(OGD+G)were randomly set in vitro.Both Western Blot and immunofluorescence staining techniques were used to detect the expression of TLR4 in cells of each group in vitro and in the left corpus callosum region in vivo.Results:The expression of TLR4 was significantly increased in OGD-induced microglia.After gastrodin intervention,TLR4 expression was decreased significantly(P＜0.05).Conclusion:GAS can inhibit the expression of TLR4 in activated microglia and thus play a neuroprotective role in HIBD.
关键词
缺血缺氧性脑损伤/天麻素/toll样受体4/氧糖剥夺/小胶质细胞/大鼠Key words
hypoxia-ischemic brain damage(HIBD)/gastrodin(GAS)/toll-like receptor 4(TLR4)/oxygen glu-cose deprivation(OGD)/microglia/rat引用本文复制引用
出版年
2024
NETL
NSTL
万方数据