广西毛冬青通过抑制氧化应激改善放射后小鼠认知功能损伤
Ilex pubescens var.kwangsiensis improved radiation-induced cognitive impairment of mice by inhibiting oxidative stress
李婷 1何改改 1孔蔺莎 1阮林 2王绍军 3韦力4
作者信息
- 1. 广西医科大学基础医学院,南宁 530021
- 2. 广西医科大学第一附属医院放疗科,南宁 530021
- 3. 广西壮族自治区妇幼保健院,南宁 530005
- 4. 广西医科大学基础医学院,南宁 530021;广西高校人体发育与疾病研究重点实验室,南宁 530021
- 折叠
摘要
目的:探讨广西毛冬青改善放射诱导的认知功能障碍及其初步机制.方法:SPF级雄性昆明小鼠,随机分为对照组(Control)、毛冬青组(IP)、放射组(Rad)、放射+毛冬青组(Rad+IP),10只/组,放射前后连续灌胃给药共14 d,Control组和Rad组给予等量生理盐水.药物干预前后采用Morris水迷宫实验和避暗实验检测小鼠认知功能变化;苏木素-伊红(HE)染色和电镜观察小鼠海马病理学变化及超微结构;免疫组织化学染色检测核因子E2相关因子2(Nrf2)、血红素氧合酶1(HO-1)、锰超氧化物歧化酶(MnSOD)、B细胞淋巴瘤-2基因(Bcl-2)和Bcl-2相关X蛋白(Bax)表达情况.结果:Rad后小鼠海马MnSOD和Bcl-2表达明显降低,Nrf2、Bax和HO-1表达明显增多,Nrf2的核易位也显著增多,广西毛冬青干预后,MnSOD表达明显升高,胞核中Nrf2核表达进一步增多,HO-1的表达水平进一步升高,凋亡率明显降低.结论:广西毛冬青改善受照射小鼠认知功能障碍,其机制可能与Nrf2激活并调控HO-1转录与表达,减轻氧化应激损伤有关.
Abstract
Objective:To investigate the improvement of radiation-induced cognitive dysfunction and its preliminary mechanism by Ilex pubescens var.kwangsiensis.Methods:SPF grade male Kunming mice were randomly divided into control group(Control),Ilex pubescens var.kwangsiensis(IP),radiation group(Rad)and radiation+Ilex pubescens var.kwangsiensiss group(Rad+IP),with 10 mice in each group.Morris water maze test and dark avoidance test were used to detect the changes in cognitive function of mice before and after drug intervention.Hematoxylin-eosin(HE)staining and electron microscopy were used to observe the changes of brain histopathology and ultrastructure in the hip-pocampus of mice.The expressions of nuclear factor E2-associated factor 2(Nrf2),heme oxygenase 1(HO-1),man-ganese superoxide dismutase(MnSOD),B-cell lymphoma/leukemia-2(Bcl-2)and Bcl-2 associated X protein(Bax)were detected by immunohistochemical assay.Results:The expressions of MnSOD and Bcl-2 in hippocampal tissue of mice in Rad group were significantly decreased,the expressions of Nrf2,Bax and HO-1 were significantly increased,and the nuclear translocation of Nrf2 was also significantly increased.After intervention,he expressions of MnSOD,Nrf2 and HO-1 were significantly increased,and the apoptosis rate was significantly decreased.Conclusion:Ilex pu-bescens var.kwangsiensis improved cognitive dysfunction in mice after radiation,and the mechanism may be related to Nrf2 activation and regulation of HO-1 transcription and expression,reducing oxidative stress damage.
关键词
广西毛冬青/放射性脑损伤/氧化应激/凋亡/认知功能Key words
Ilex pubescens var.kwangsiensis/radiation brain injury/oxidative stress/apoptosis/cognitive function引用本文复制引用
基金项目
国家自然科学基金(81460479)
广西自然科学基金(2018JJA140600)
广西自然科学基金(2023JJA141166)
出版年
2024
NETL
NSTL
万方数据